As a typical remediation and enhancement ecosystem, artificial reefs are manipulated to some extent in terms of their biological community structure and ecological environment. Therefore, carbon circulation and carbon-fixation of the reef system are greatly influenced by production activities of human beings. In this study, mechanism of carbon sink of main carbon-fixing biological factors is analyzed according to the structure feature of artificial reefs. Methods for calculating fixed carbon in reef area are discussed, and approaches for promoting carbon-fixing capacity of marine organism through artificial reef construction are analyzed.