文章摘要
大菱鲆( Scophthalmus maximus )水通道蛋白(AQP1、AQP3)以及离子通道蛋白(CFTR、NHE1)基因对低盐胁迫的响应
Response of aquaporin (AQP1, AQP3) and ion channel protein (CFTR, NHE1) genes of turbot (Scophthalmus maximus) to low-salinity stress
投稿时间:2019-04-10  修订日期:2019-06-03
DOI:
中文关键词: 大菱鲆  低盐胁迫  水通道蛋白  囊性纤维化跨膜传导调节因子  Na+/H+-exchanger  荧光定量PCR
英文关键词: Scophthalmus maximus  Low-salinity stress  Aquaporins  Cysticfibrosis transmembrane regulator  Na+/H+-exchanger  Quantitative real-time PCR
基金项目:现代农业产业技术体系建设专项(CARS-47-01);中国水产科学研究院黄海水产研究所基本科研业务费(20603022019019);青岛海洋科学与技术国家实验室“鳌山人才”培养计划项目(2017ASTCP-OS04);国家自然科学基金项目(41706168);山东省农业良种工程项目(2016LZGC031)
作者单位E-mail
张金生 中国水产科学研究院黄海水产研究所 1451980580@qq.com 
马爱军 中国水产科学研究院黄海水产研究所 maaj@ysfri.ac.cn 
刘志峰 中国水产科学研究院黄海水产研究所  
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中文摘要:
      盐度是影响鱼类生长代谢等各种生理活动的重要环境因素,在鱼类适应环境盐度变化的过程中,鳃、肾、肠是主要的渗透调节器官,而水通道蛋白(aquaporins,AQPs)、囊性纤维化跨膜传导调节因子(CFTR)、Na+/H+-exchanger(NHE)又是这些器官中重要的渗透调节基因。为研究AQP1、AQP3、CFTR、NHE1在大菱鲆( Scophthalmus maximus )低盐胁迫过程中的渗透调节功能,本研究采用荧光定量PCR技术对四种基因在5盐度和10盐度下大菱鲆鳃、肾、肠中表达量随时间的变化进行检测。结果显示,AQP1表达量在鳃中极少(P<0.05),在肾和肠中较高,低盐胁迫下,5盐度组和10盐度组在鳃中的表达量无显著变化,在肾和肠中均显著上升(P<0.05)。AQP3表达量在肾中极少(P<0.05),在鳃中较高,在肠中较少,低盐胁迫下,5盐度组和10盐度组在肾中的表达量无显著变化,在鳃和肠中均显著上升(P<0.05)。CFTR表达量在肾中极少,在鳃中较高,在肠中较少,低盐胁迫下,5盐度组和10盐度组在肾中的表达量无显著变化,在鳃和肠中均显著下降(P<0.05)。NHE1在鳃和肠中表达量较少,在肾中较高,低盐胁迫下,5盐度组和10盐度组在鳃中的表达量无显著变化,在肾和肠中均显著上升(P<0.05)。这些结果表明四种基因表达水平因组织、盐度和时间的不同而不同,反映了这四种基因的功能特异性;在低盐胁迫下,四种基因积极响应,表达量均发生不同程度变化,表明AQP1、AQP3、CFTR和NHE1在大菱鲆低盐环境适应中可能具有潜在的重要作用。另外本研究的实验结果可为大菱鲆半咸水养殖和淡化养殖提供理论依据,同时为培育适应低盐环境大菱鲆良种提供理论和技术支撑。
英文摘要:
      Salinity is an important environmental factor affecting the growth and metabolism of fish. Gill, kidney and intestine are main osmotic regulation organs in the process of fish adapting to environmental salinity changes, while aquaporins(AQPs), Cysticfibrosis transmembrane regulator (CFTR)and Na+/H+-exchanger(NHE) are important osmoregulatory genes in these organs. In order to study the osmotic regulation function of AQP1, AQP3, CFTR and NHE1 in turbot (Scophthalmus maximus) under low-salinity stress, the expression levels of four genes in gill, kidney and intestine of turbot at 5 salinity and 10 salinity were detected by Quantitative real-time PCR. The results showed that the expression level of AQP1 was very low in gill, but high in kidney and intestine. Under low-salinity stress, the expression of AQP1 in gill did not change significantly in 5 salinity group and 10 salinity group, but increased significantly in kidney and intestine (P<0.05). The expression level of AQP3 was very low in kidney, with high in gill and low in intestine. Under low-salinity stress, the expression level of AQP3 in kidney did not change significantly in 5 salinity and 10 salinity groups, but increased significantly in gill and intestine (P<0.05). The expression level of CFTR was very low in kidney, with high level in gill and low level in intestine. Under low-salinity stress, the expression level of CFTR in kidney did not change significantly in 5 salinity and 10 salinity groups, but decreased significantly in gill and intestine (P<0.05). NHE1 was less expressed in gill and intestine, but high in kidney. Under low-salinity stress, the expression of NHE1 in gill did not change significantly in 5 salinity group and 10 salinity group, but increased significantly in kidney and intestine (P<0.05). These results indicate that the expression levels of the four genes vary with tissue, salinity and time, reflecting the functional specificity of the four genes. Under low-salinity stress, the four genes responded positively and the expression levels changed to varying degrees, indicating that AQP1, AQP3, CFTR and NHE1 may have potential roles in the adaptation of turbot low salt environment. In addition, the results could provide theoretical basis for brackish water aquaculture and desalination aquaculture of turbot, and also provide theoretical and technical support for breeding improved turbot varieties adapted to low-salinity environment.
相关附件:   基因表达图  万方20190410(10.23%)  第一审稿人评审意见  第二审稿人评审意见  第一审稿人复审意见  AQP1相对表达量修改图  AQP3相对表达量修改图  CFTR相对表达量修改图  NHE相对表达量修改图
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