文章摘要
泥东风螺EST-SSR开发及其群体遗传多样性分析
Development and Genetic Diversity Analysis of the EST-SSR Markers in Babylonia lutosa
投稿时间:2019-04-17  修订日期:2019-05-12
DOI:
中文关键词: 泥东风螺(Babylonia lutosa)  转录组  EST-SSR  遗传多样性
英文关键词: Babylonia lutosa  Transcriptome  EST-SSR  Polymorphism
基金项目:国家自然科学基金(31672640)、湖南省自然科学基金(2017JJ3134/2016NK2115)、湖南省教育厅基金项目(17C0935)
作者单位E-mail
熊 钢 湖南生物机电职业技术学院 动物科技系 xionggang709@126.com 
王晓清① 湖南农业大学 动物科技学院 wangxiao8258@126.com 
王佩 湖南农业大学 动物科技学院  
陈贞年 湖南农业大学 动物科技学院  
周先文 湖南农业大学 动物科技学院湘西州水产工作站
湘西州水产工作站
 
 
.康 骊 湖南生物机电职业技术学院  
曾志南 福建省水产研究所  
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中文摘要:
      本文采用MISA软件分析泥东风螺(Babylonia lutosa)转录组中微卫星信息。结果显示,从转录组中共获得16324个SSR,共有181种重复基元;泥东风螺转录组中不同类型微卫星的重复基序具有不同的分布特征,其中,二核苷酸重复基元中AC/GT(70.58%)重复基元以重复6次出现频率占优;长度为12~20 bp的SSR占63.95%,长度为21~25 bp的SSR占9.14%,总体的平均长度为18.4 bp。随机选取其中50条序列设计引物,通过对福建野生泥东风螺群体(WP)DNA样本进行PCR扩增和分型,结果获得23个多态性位点,等位基因数目为2~7个不等,期望杂合度(Ne)为0.190~0.937,观察杂合度(No)为0.065~0.936, 多态性信息含量(PIC)为 0.061~0.777,有4个位点显著偏离HWE平衡(P<0.05)。对野生群体和养殖群体(BP)遗传多样性分析显示野生群体和养殖群体的平均期望杂合度分别为0.491和0.544,平均观察杂合度分别为0.477和0.564,平均多态性信息含量分别为0.541和0.407。Fis结果显示野生群体和养殖群体分别有13个和9个位点杂合子过剩。群体间遗传分化指数(Fst)为0.001~0.655,平均值为0.053(0.05<遗传分化指数<0.15),属于中等程度分化。群体间的基因流值(Nm)为0.132~543.787,平均为4.450。两群体间的遗传距离为0.892,表明群体间的遗传分化小。本研究结果表明泥东风螺野生群体和养殖群体均具有较高的遗传多样性,具有很大的选育潜力,开发的泥东风螺SSR标记对泥东风螺的群体遗传结构、图谱构建、增殖放流效果评估和分子辅助育种等研究方面具有重要的意义。
英文摘要:
      In this study, We analyzed the information characteristics of the Babylonia lutosa`s microsatellites after obtained transcriptome sequences with MISA software.The research results show that a total of 16,342 microsatellites were identified, and 181 nucleotide repeat motifs were identified. Different types of repeat microsatellites had considerably different distribution characteristics. Mononucleotide and Dinucleotide microsatellite repeating units were the most abundant in B. lutosa transcriptome, in which the 6 repeats of AC/GT (70.58%) were the dominant repeating units of Dinucleotide. The length of dominant repeating units were 12~20 bp (63.95%) and 21~25bp(9.14%), respectively, and the average length is 18.4 bp. Among the 50 designed primer pairs, 23 pairs were proved to be polymorphic microsatellite markers in the B. lutosa wild populations (WP). The result showed that the allele number of these microsatellites ranged from 2 to 7. the expected heterozygosity (He) and observed heterozygosity (Ho) ranged from 0.190 to 0.937 and 0.065 to 0.936, respectively. The polymorphism information content (PIC) ranged from 0.061 to 0.777. The He values of WP and breed population (BP) were 0.491 and 0.544, respectively. The Ho values of WP and BP were 0.477 and 0.564, respectively. The PIC values of WP and BP were 0.541 and 0.407, respectively. There were 13 population loci that was heterozygote excess in WP, and 13 population loci in BP. The genetic differentiatio indes (Fst) was ranged from 0.001 to 0.655, with an average value of 0.053(0.05<Fst<0.15t ), and the gene flow value (Nm) ranged from 0.132 to 543.787, with an average value of 4.450. The genetic distance between the WP and BP was 0.892, which indicated that two populations generated?little genetic differentiation.This results above show that the BP still has high genetic diversity after selection, and it also has potential value for further breeding materials. The EST-SSR in this study will facilitate further studies on the population genetic structure, genetic mapping, evaluation artificial?releasing and molecular assisted breeding of B. lutosa.
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