文章摘要
基于简化基因组测序的中国对虾3个选育世代遗传多样性分析
Analysis of Genetic Diversity in Three Generations Breeding Populations of Fenneropenaeus chinensis 2b-RAD sequencing
投稿时间:2019-05-10  修订日期:2019-05-30
DOI:
中文关键词: 中国对虾  选育群体  2b-RAD  SNP  遗传多样性
英文关键词: Fenneropenaeus chinensis  Breeding Populations  2b-RAD  SNP  Genetic diversity
基金项目:
作者单位E-mail
王凤娇 上海海洋大学 1730585520@qq.com 
孟宪红 中国水产科学研究院黄海水产研究所 mengxianhong@ysfri.ac.cn 
傅强 中国水产科学研究院黄海水产研究所  
栾生 中国水产科学研究院黄海水产研究所  
隋娟 中国水产科学研究院黄海水产研究所  
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中文摘要:
      本研究基于简化基因组测序(2b-RAD)技术,对中国对虾“黄海2号”2015-2017 年3个连续选育世代(G9-G11)的亲本群体、共649个个体进行了简化基因组测序,并对这3个亲本群体进行了遗传结构和遗传多样性分析。实验在三个选育世代中共获得66,985个SNP位点。遗传分析的结果显示,G9-G11平均核苷酸多样性(Pi)分别为0.1439、0.1587和0.1674,平均观测杂合度(Ho)分别为0.1388、0.1515和0.1609,多态信息含量(PIC)分别为0.1241、0.1360和0.1430。G9-G11亲本群体的遗传多样性整体呈现一定的上升趋势,但差异不显著。F-检验显示,3个世代总的Fst值为0.0061,G9-G11相邻世代群体间遗传分化程度较弱(G9-G10为0.0029,G10-G11为0.0026)。相邻世代的遗传距离逐渐减小,分别为0.0029、0.0026。3个世代间基因交流充分,基因流为62.91-94.63。本研究结果表明,人工定向选育工作的推进对中国对虾选育群体遗传多样性和遗传结构产生了一定的影响:在固定的选择压力下(4%-5%),亲本群体的遗传多样性并无降低的趋势,中国对虾选育群体遗传分化相当小,遗传结构趋向稳定。研究结果为进一步制定中国对虾选育计划提供基础遗传数据和科学的理论指导。
英文摘要:
      Based on a reduced-representation genome sequencing (2b-RAD) technique, this study performed a genome-wide SNP screening in 649 individuals selected from three consecutive breeding generations (G9-G11) in a population of Fenneropenaeus chinensis named Huanghai 2". Genetic structure and genetic diversity of the parents’ population were further analyzed using these markers. Totally, 66,985 SNPs were developed and genotyped in all 649 shrimps. The results of genetic analysis showed that the average nucleotide diversity (Pi) was 0.1439, 0.1587 and 0.1674 in G9-G11. The average observed heterozygosity (Ho) were 0.1388, 0.1515 and 0.1609, respectively. The polymorphic information content (PIC) was 0.1241, 0.1360 and 0.1430. Genetic diversity parameters of the G9-G11 parent population showed an undisputed upward trend, although not significant. F-test showed that the total Fst value of all three generations was 0.0061, and the degree of genetic differentiation between each adjacent generations was weak (G9-G10 0.0029, G10-G11 0.0026). The genetic distances of adjacent generations decreased slightly, which were 0.0029 and 0.0026 in G9-G10 and G10-G11, respectively., The gene flow was 62.91-94.63 in all generations, which indicated that the gene exchange was sufficient. Results of this study confirmed the certain impact of artificial selection on genetic diversity and genetic structure in Fenneropenaeus chinensis breeding populations. It also showed that our breeding strategies (selection pressure was 4%-5% in each generation), do not produce bad effects on its genetic diversity in parental populations. The genetic differentiation in each generation of Fenneropenaeus chinensis breeding population is quite small, and the genetic structure tends to be stable. The results of the study provide a basic database for genetic analysis in Fenneropenaeus chinensis from molecular level, and also provide theoretical guidance and data support for the formulation of breeding program in Fenneropenaeus chinensis.
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