文章摘要
UV-B紫外辐射对条斑紫菜(Pyropia yezoensis)生理生化的影响
Effects of UV-B ultraviolet radiation on the physiology and biochemistry of Pyropia yezoensis
投稿时间:2019-05-13  修订日期:2019-05-31
DOI:
中文关键词: 条斑紫菜  UV-B  光合作用  叶绿素荧光  酪氨酸酶
英文关键词: Pyropia yezoensis  UV-B  Photosynthesis  Chlorophyll fluorescence  Tyrosinase
基金项目:山东省支持青岛海洋科学与技术试点国家实验室重大科技专项
作者单位E-mail
潘雪 中国海洋大学海洋生物遗传学与育种教育部重点实验室 545166507@qq.com 
茅云翔 中国海洋大学海洋生物遗传学与育种教育部重点实验室  
王俊皓 中国海洋大学海洋生物遗传学与育种教育部重点实验室  
曹敏 中国海洋大学海洋生物遗传学与育种教育部重点实验室  
毕桂萁 中国海洋大学海洋生物遗传学与育种教育部重点实验室  
杜国英① 中国海洋大学海洋生物遗传学与育种教育部重点实验室 duguo923@ouc.edu.cn 
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中文摘要:
      条斑紫菜(Pyropia yezoensis)作为一种生长在潮间带的经济海藻,在适应干露失水时的高紫外辐射上有其独特的特性。本实验以无UV-B辐射为对照组,添加0.5 W/m2和2 W/m2的低和高UV-B辐射为实验组,检测条斑紫菜对UV-B紫外辐射响应的叶绿素荧光光合参数、多光谱荧光参数以及酪氨酸酶活性的变化。结果显示,受到UV-B辐射的条斑紫菜其叶绿素荧光光合参数(QY_max、NPQ_Lss、qP_Lss、Rfd_Lss)随辐照时间呈现不同程度的下降趋势,并且高辐射强度下的各光合参数值低于低辐射强度的,表明条斑紫菜光合作用受到辐射的负面影响;极早期胁迫参数F440/F690和F440/F740的差异表明2 W/m2 UV-B辐射组条斑紫菜比0.5 W/m2辐照组更早(1 h)进入胁迫状态,且UV-B辐射下条斑紫菜酚醛类物质减少;与叶绿素浓度呈负相关的参数F690/F740变化表明,0.5 W/m2辐照组叶绿素浓度高于对照组,而在辐照3h内低于2 W/m2辐照组,2 W/m2辐照组叶绿素浓度则先上升并在辐照4 h下降至6h呈显著降低,说明藻体可耐受较低UV-B辐射,而高辐射UV-B长时间辐照下对藻体造成明显的伤害;对酪氨酸酶的活性检测结果表明,受到UV-B辐照的条斑紫菜酪氨酸酶活性显著高于对照组,且高辐照组的酶活性高于低辐照组的酶活性。
英文摘要:
      Abstract: As a kind of economic algae growing in the intertidal zone, Pyropia yezoensis has its unique characteristics on adapting to the high ultraviolet radiation in open air and desiccation conditions. In this experiment, the light without UV-B radiation was taken as control, and low UV-B radiation of 0.5 W/m2 and high UV-B radiation of 2 W/m2 was added to detect the changes of chlorophyll fluorescence photosynthetic parameters, multi-spectral fluorescence parameters and tyrosinase activity in response to UV-B ultraviolet radiation of gametophytes of Pyropia yezoensis. The results showed that the chlorophyll fluorescence photosynthetic parameters (QY_max, NPQ_Lss, qP_Lss, Rfd_Lss) of P. yezoensis with UV-B radiation displayed a gradient decline with irradiation time, and the values of photosynthetic parameters were lower at high radiation intensity (2 W/m2) than those under low radiation intensity (0.5 W/m2). The early stress parameters of F440/F690 and F440/F740, which positively related to the secondary metabolites such as phenol or aldehyde concentration, showed that the group under the condition of 2 W/m2 entered a forced state earlier than the group under the condition of 0.5 W/m2. The secondary metabolites such as phenol or aldehyde concentration decline The F690/F740, which was negatively correlated with the chlorophyll concentration, showed that the chlorophyll concentration under 0.5 w/m2 irradiation was higher than that of under no UV-B irradiation, but lower than the maximum value in the rising period of 2 W/m2 irradiation. The chlorophyll concentration increased at first and decreased rapidly after 4 h under the condition of 2 W/m2, it indicated that the chlorophyll concentration is less sensitive to UV-B radiation under low radiation intensity, whereas it's higher. The results showed that the tyrosinase activity of P. yezoensis gametophytes irradiated with UV-B was significantly higher the control group, and the enzyme activity under 2 W/m2 UV-B was higher than 0.5 W/m2 UV-B.
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