文章摘要
中国鲎人工繁育及1龄稚鲎形态性状对体质量的影响分析
Effects of Morphological Characters on Body Weight of Artificially Bred the first instars of Horseshoe Crabs Tachypleus tridentatus
投稿时间:2019-05-15  修订日期:2019-06-04
DOI:
中文关键词: 中国鲎  人工繁育  形态性状  相关分析  通径分析
英文关键词: Tachypleus tridentatus  artificial propagation  morphological trait  correlation analysis  path analysis
基金项目:中央级公益性科研院所基本科研业务费专项基金
作者单位E-mail
鲍虞园 中国水产科学研究院南海水产研究所 answerbyy@sina.com 
叶国玲 中国水产科学研究院南海水产研究所  
颉晓勇 中国水产科学研究院南海水产研究所 xyxie@scsfri.ac.cn 
摘要点击次数: 129
全文下载次数: 0
中文摘要:
      2018年5月 ~ 9月在广东湛江对中国鲎进行人工繁育技术初步研究。在水温为28~32℃,盐度为26~30,pH为7.4~7.8的条件下, 9对中国鲎共计产卵8.713×104粒,受精率为56%~68%;受精卵经历约45天胚胎发育孵化成1龄稚鲎,孵化率达到92%,共计获得1龄稚鲎4.827 万尾。随机取样人工繁育1龄中国鲎500尾,测量头胸甲长(X1)、头胸甲宽(X2)、头胸甲高(X3)、腹部长(X4)和体质量(Y )5个形态性状,应用相关分析、多元回归、通径分析、决定系数分析等方法,分别计算得出各个形态性状对体质量(Y )的相关系数、通径系数以及决定系数,进一步分析形态性状对体质量的直接作用和间接作用相关分析。结果表明,所有形态性状与体质量的相关系数均达到极显著水平(P<0.01)。各形态性状对于体质量的通径系数均达到极显著水平(P<0.01),其中通径系数最大的是头胸甲宽,说明头胸甲宽对体质量的直接作用最大。决定系数分析结果与通径分析结果一致,各表型性状对体质量的总决定系数Σd = 0.863,表明所选形态性状是影响体质量的主要性状。通过逐步回归分析方法,经偏回归系数显著性检验,建立了以体质量为因变量,头胸甲长、头胸甲宽、头胸甲高和腹部长为自变量的多元回归回归方程: Y = -0.228 + 0.248X1 + 0.073X2 + 0.333X3 + 0.073X4。结论:1龄中国鲎的头胸甲宽对体质量的影响最大,种质评价和放流苗种筛选应以头胸甲宽度为首选依据。
英文摘要:
      Tachypleus tridentatus is a rare marine living fossil with immense economic value and scientific research value. However, in recent years, over fishing and indiscriminate killing have led to a sharp decline in the number of horseshoe crabs. Artificial release proliferation is not only an important way to recover horseshoe crab, but also protect biodiversity and promote sustainable development of the fishery. We conducted a preliminary study on artificial breeding technology of horseshoe crabs T. tridentatus in Zhanjiang, Guangdong Province from May to September 2018. When the water temperature is 28~32℃, the salinity is 26~30, and the pH is 7.4~7.8, 9 pairs of T. tridentatus laid 87130 eggs in total, with a fertilization rate of 56% to 68%. After 45 days, the embryo hatches into the first instars of the juvenile T. tridentatus, the hatching rate reaches 92%, and a total of 48,268 T. tridentatus larvae were obtained. In order to study the correlation between phenotypic traits and body weight, five quantitative traits (carapace length X1, carapace width X2, carapace height X3, abdomen length X4, and body weight Y) of the first instars of T. tridentatus were measured and analyzed from 500 randomly collected individuals by correlation analysis, multiple regression analysis, path analysis and determination coefficient analysis. The results showed that there were considerable correlations (P<0.01) among the five measured traits. T Path coefficients of various morphological traits for body mass reached a very significant level (P<0.01), and the largest path coefficient was the width of the head and chest armor, which indicated that the width of the head and chest armor had the greatest direct effect on body mass. The trend of determination coefficient analysis was comparable to that of path analysis. The elevated total determination coefficient (Σd=0.863) between phenotypic features and body weight indicated that the selected traits were practically useful. The multiple regression equation for estimation of body weight was Y = -0.228 + 0.248X1 + 0.073X2 + 0.333X3 + 0.073X4. Conclusion: The carapace width of the first instars of T. tridentatus has the greatest influence on body mass. Therefore, the width of cephalothorax should be the first choice for germ plasm evaluation and seedling screening.
相关附件:   万方20190515(22.11%)  审稿意见问题回复及稿件修改明细  形态指标测量示意图
View Fulltext   查看/发表评论  下载PDF阅读器
关闭