文章摘要
半滑舌鳎hsd11b1l和hsd11b2基因的克隆及其温度响应的表达规律*
Molecular characterization and expression patterns of hsd11b1l and hsd11b2 and their response to high temperature stress in Chinese tongue sole (Cynoglossus semilaevis)
投稿时间:2020-03-12  修订日期:2020-03-20
DOI:
中文关键词: 半滑舌鳎  性别决定  温度  hsd11b1l基因  hsd11b2基因
英文关键词: Chinese tongue sole  sex determination  temperature  hsd11b1l  hsd11b2
基金项目:
作者单位E-mail
郝先才 上海海洋大学水产与生命学院 上海 best_hxc@163.com 
冯博 上海海洋大学水产与生命学院 上海  
邵长伟 上海海洋大学水产与生命学院 上海  
王倩 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 wangqian2014@ysfri.ac.cn 
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中文摘要:
      鱼类的性别决定和分化不仅受到遗传因素的控制,同时也受到环境因素的影响。皮质醇在鱼类应对外界环境压力的过程中起到重要调控作用,而hsd11b1l和hsd11b2具有调节体内皮质醇浓度的重要功能。本研究克隆了半滑舌鳎(Cynoglossus semilaevis) hsd11b1l和hsd11b2基因的cDNA全长序列,分析了其序列特征,研究了其时空表达特征及温度响应的表达规律。结果显示,hsd11b1l cDNA全长为1650 bp,开放性阅读框长度为864bp,编码287个氨基酸;hsd11b2 cDNA全长为4526 bp,开放性阅读框长度为1209 bp,编码402个氨基酸。半滑舌鳎不同组织和性腺发育时期的表达分析结果表明,hsd11b1l在肝脏中表达量最高,卵巢的表达量是精巢的两倍,且在6月龄和3龄鱼的卵巢中呈现较高表达;而hsd11b2主要在精巢中表达,在6月龄鱼的精巢中表达量最高,随后表达量急剧降低,在卵巢中各个时期几乎不表达。半滑舌鳎温度响应的表达结果显示高温(28°C)处理两个月后,与正常温度(22°C)对照组相比,hsd11b1l和hsd11b2在雄鱼中的表达量都显著降低(p<0.05);高温短期应激48小时,hsd11b1l在雌鱼和雄鱼中均显著降低表达, hsd11b2表达仅在雄鱼中有显著性下调(p<0.05)。本研究探讨了hsd11b1l和hsd11b2基因在半滑舌鳎性别分化过程中的表达规律,为研究环境温度与半滑舌鳎性别分化之间的关系奠定了基础。
英文摘要:
      Sex determination and differentiation in fish are not only influenced by genetic factors, but also controlled by environmental factors. Previous studies have shown that cortisol plays an important role in feedback of environmental stress in fish and the hsd11b1l and hsd11b2 can regulate the concentration of cortisol. In this study, we cloned the full-length cDNA of hsd11b1l and hsd11b2 and analyzed their sequence characteristics in Chinese tongue sole (Cynoglossus semilaevis). Then, we detected their spatiotemporal expression characteristics and expression patterns after temperature stress conditions. The full-length cDNA of hsd11b1l was 1650 bp long with 864bp open reading frame encoding a predicted 287 amino acid protein. While the full-length of hsd11b2 was 4526 bp longs with 1209bp open reading frame encoding 402 amino acid protein. qPCR shows that the hsd11b1l was highest expressed in liver and the expression level in ovary was two-fold than in testis. In particularly, the expression level of hsd11b1l in ovary was higher than in testis at the stages of 6 mph and 3 yph. The hsd11b2 was expressed mainly in testis and its expression level was reached to a peak in testis at 6 mph; Conversely, its expression was hardly detected in the stages of ovary development. In addition, we analyzed the expression patterns of hsd11b1l and hsd11b2 after high-temperature (28°C) treatment. The expression levels of hsd11b1l and hsd11b2 was significantly reduced in the gonads of male (p<0.05) after the high-temperature treatment for two months. For the acute high-temperature treatment (48h), the expression of hsd11b1l was significantly decreased in the gonads both of female and male (p<0.05), and the expression of hsd11b2 was significantly down-regulated only in the testis of male (p<0.05). In this study, the results of the expression patterns of hsd11b1l and hsd11b2 genes in the developmental stages of gonads and the high temperature treatment lays a foundation for understanding the relationship between temperature and sexual differentiation in Chinese tongue sole.
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