文章摘要
盐度对花鲈幼鱼鳃、脾及肌肉组织结构的影响
Effect of salinity on histological structure of gills, spleen and muscle in juvenile Lateolabrax maculatus
投稿时间:2018-11-01  修订日期:2018-12-10
DOI:
中文关键词: 盐度  花鲈    脾脏  肌肉  组织结构
英文关键词: salinity  Lateolabrax maculatuswas  gills  spleen  muscle  histological structure
基金项目:中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2016TS02)
作者单位E-mail
温久福 中国水产科学研究院南海水产研究所 nhswjf@163.com 
蓝军南 中国水产科学研究院南海水产研究所,上海海洋大学水产与生命学院  
曹明 广东省渔业种质保护中心  
周慧 中国水产科学研究院南海水产研究所  
区又君 中国水产科学研究院南海水产研究所 ouyoujun@126.com 
李加儿 中国水产科学研究院南海水产研究所  
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中文摘要:
      为了探究不同盐度条件花鲈幼鱼组织结构的变化特点,用组织学方法对不同盐度下(S0、S10、S15、S20、S30)花鲈幼鱼的鳃、脾及肌肉组织结构进行研究。结果显示,S0条件下,花鲈幼鱼鳃丝排列紧密,顶端膨大呈棒状,鳃小片细胞饱满,有较多氯细胞。花鲈幼鱼鳃丝宽度随盐度的升高而缩小,鳃小片间距则逐渐增大,差异显著(P<0.05);S20条件下,部分鳃小片变形脱落,鳃丝上的泌氯细胞明显增多增大;在S30条件下,鳃丝宽度较大,出现断裂脱落,鳃丝上细胞排列疏松,泌氯细胞明显膨大,有溶解现象。。脾脏在淡水条件下(S0),淋巴细胞数目较少,血细胞较多;在低盐环境中(S10、S15),淋巴细胞增大,数量增多,黑色素巨噬细胞中心数量增加;在高盐度下(S30),脾细胞和部分淋巴细胞出现肿大、空泡化现象,细胞排列疏松。S0条件下,花鲈幼鱼肌纤维排列较为疏松,多角形或长椭圆形,长径和短径较大,密度较小;S15条件下,肌纤维短径变小,密度增大,与S0、S10组均差异显著,S30条件下,肌纤维长径增大,密度减小;随着盐度的增加,肌纤维长径和短径均有先减小后增加的趋势,单位面积肌纤维数量则先增加后减小,差异显著(P<0.05)。实验结果表明,花鲈幼鱼鳃、脾及肌肉组织结构变化特征与其所处的环境盐度有关。
英文摘要:
      In order to explore the characteristics of histological structure of Lateolabrax maculatus juveniles cultured at different salinity, the histological structure of gill, spleen and muscle under different salinities (S0、S10、S15、S20、S30) were observed by using the histological methods. The results showed that, under S0 condition, the gill filaments of L. maculatus juveniles closely arranged closely, the top of the gill expanded into a rod shape, and the gill cells were plump with more chlorine cells; the width of gill filament decreased and the intervals of gill lamella became larger with the increase of salinity obviously(P<0.05); at S20 condition, some gill lamellas decomposed and falled off; the number of chlorine secreting cells on gill filaments increased significantly; the number and size of chloride cells increased with salinity rising, some chloride cells expanded and dissolved under the condition of S30. In fresh water, more blood cells and fewer lymphocytes were observed in spleen, while in the hyposaline environment (S10, S15), lymphocytes were enlarged, and the number of melanin macrophages increased. At high salinity (S30), the spleen cells and some lymphocytes showed swelling, vacuolation and arranged loosely. Under S0 condition, the muscle fibers of juveniles were loosely arranged, polygonal or oblong, with larger major and minor diameters and smaller density. With the increase of salinity, both the length and short diameter of muscle fiber tended to decrease firstly and then increase again, at S15 condition, the short diameter of muscle fiber became smaller and its density increased, which it was significantly different from that of S0 and S10. Under S30 condition, the long diameter of muscle fiber increased and its density decreased. In summary, as salinity increased, both the long and short diameter of muscle fibers tended to decrease first and then increase (P<0.05). The results indicated that the histological structure of gill, spleen and muscle of L. maculatus juveniles were affected by environment and salinity.
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