文章摘要
复合蛋白替代鱼粉对花鲈生长、消化能力和肠道健康的影响
Effects of replacing fish meal with composite protein on growth, digestive enzymes activities and gut health in Japanese seabass (Lateolabrax maculatus)
投稿时间:2018-12-14  修订日期:2019-01-30
DOI:
中文关键词: 复合蛋白,花鲈, 生长,消化,肠道组织结构,炎性因子
英文关键词: Composite protein  Japanese seabass (Lateolabrax maculatus)  Growth performance  Digestibility  Gut morphology structure  Inflammatory genes[Corresponding Author: ZHANG Chunxiao, E-mail: cxzhang@jmu.edu.cn]
基金项目:国家海水鱼产业技术体系(CARS-47)
作者单位E-mail
胡鹏莉 集美大学水产学院 970260221@qq.com 
张春晓 集美大学水产学院 cxzhang@jmu.edu.cn 
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中文摘要:
      以初均重5.80±0.07 g的花鲈幼鱼为试验对象,研究复合蛋白替代鱼粉对花鲈生长、消化和肠道健康的影响。用复合蛋白分别替代正常饲料中0%、20%、40%、60%和80%的鱼粉,配制成5种等氮的试验饲料,分别用这5种饲料投喂花鲈70天;养殖试验期间水温29.5±1.5°C,盐度29±1‰,溶解氧6.5±1mg/L。养殖试验结束后采集样品进行分析,结果表明:20%替代组花鲈生长性能与对照组无显著差异(P >0.05),但随鱼粉替代比例的升高,花鲈增重率、存活率、饲料效率以及蛋白质效率显著下降(P<0.05),花鲈肠道蛋白酶活性以及饲料干物质、粗蛋白与粗脂肪表观消化率皆显著降低(P<0.05),且花鲈后肠黏膜组织结构出现损伤,肠道促炎因子TNF-α、IL-1β基因表达量升高,抗炎因子基因IL-4、IL-10表达量相应降低。综上所述,复合蛋白替代花鲈饲料中鱼粉比例不宜超过20%。
英文摘要:
      Abstract This study was conducted to evaluate the effects of replacing fish meal with composite protein on growth performance, diet digestibility, and gut health in Japanese seabass (Lateolabrax maculatus). Five isonitrogenous and isolipidic diets were formulated by replacing 0, 20, 40, 60 or 80% of fish meal in basic diet with composite protein. Quadruplicate groups of 30 fish (initial body weight 5.80±0.07 g) were fed the test diets to apparent satiation twice daily (08:00 and 16:30), and the experiment continued for 70 days. During the feeding trial, water temperature, salinity and dissolved oxygen values were maintained at 29.5±1.5 °C, 29±1‰, and ≥6.5 mg/L, respectively. Samples were collected for analysis after the feeding trial. The main indexes including the growth performance, diet digestibility, intestinal digestive enzymes activities, posterior intestine morphology structure and inflammation-related genes expression of proximal intestine, mid-intestine and posterior intestine were analyzed to investigate the proper replacement proportion of fish meal by composite protein. The results showed that substituting for 20% of fish meal with composite protein did not significantly affect growth performance compared to the control group (P>0.05), while further increment of fish meal replacement level resulted in significant reduction of weight gain, survival rate, feed efficiency and protein efficiency ratio (P<0.05). Moreover, as the replacement level of fish meal with composite protein exceed 20%, intestinal protease activity and apparent digestibility coefficients (ADCs) of feed dry matter, crude protein, and crude lipid also significantly decreased with increasing fish meal replacement level by composite protein (P<0.05). Meanwhile, increasing replacement of fish meal with composite protein damaged posterior intestine morphology structure, and up-regulated gut inflammatory genes expression such as TNF-α, IL-1β, while an opposite trend was observed for anti-inflammatory genes expression of IL-4 and IL-10 genes. In conclusion, it was recommended that the replacement proportion of fish meal with composite protein should not exceed 20%.
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