文章摘要
一种复合益生菌对凡纳滨对虾抗副溶血弧菌弧菌感染能力的影响
Effect of a compound probiotics on the ability of Penaeus vannamei to resist Vibrio parahaemolyticus infection
投稿时间:2019-03-17  修订日期:2019-04-09
DOI:
中文关键词: 凡纳滨对虾  复合益生菌  副溶血弧菌  免疫基因  免疫酶
英文关键词: Penaeus vannamei  compound probiotics  Vibrio parahaemolyticus  immune genes  immune enzymes
基金项目:1.中国水产科学研究院基本科研业务费专项(2017HY-ZD0502; 2017HY-ZD1002),现代农业产业技术体系建设专项资金(CARS--47)2.中国水产科学研究院基本科研业务费专项(2017HY-ZD0502; 2017HY-ZD1002)
作者单位E-mail
杨运楷 黄海水产研究所 891293238@qq.com 
宋晓玲 黄海水产研究所 songxl@ysfri.ac.cn 
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中文摘要:
      本研究选择一株地衣芽孢杆菌(Bacillus licheniformis) BL-9、一株枯草芽孢杆菌(Bacillus subtilis) BS-12、一株金丽假交替单胞菌(Pseudoalteromonas flavipulchra) CDM8,以1:1:1将其组成复合益生菌,进行一个为期30天的凡纳滨对虾(Penaeus vannamei)养殖实验。实验分为暂养期(7天)、益生菌浸泡期(15天)、副溶血弧菌( Vibrio parahaemolyticus)攻毒期(10天)。实验结果证实养殖水体中添加复合益生菌能够显著增加水体和对虾肠道可培养细菌总数(P<0.05),攻毒实验结束时,实验组对虾累积存活率为73.3%,显著高于阳性对照组(25.3%)。对虾非特异性免疫基因热激蛋白70 (heat shock proteins 70, Hsp70)、β-1, 3-葡聚糖结合蛋白-脂蛋白(beta-1, 3-glucan-binding protein-lipoprotein, βGBP-HDL)、脂多糖-β-1,3-葡聚糖结合蛋白(lipopolysaccharide-β-1,3-glucan binding protein,LGBP)、溶菌酶(Lysozyme, LZM)、抗菌肽Crustin在益生菌浸泡阶段均出现不同程度的上调,在攻毒阶段则呈现各自不同的表达情况。研究结果提示水体添加芽孢杆菌和假交替单胞菌组成的复合益生菌可提高对虾抗副溶血弧菌感染能力,对虾抗病力的提高可能与益生菌在对虾体内定殖,抗病相关基因及酶活表达水平有关。
英文摘要:
      In this 30-days culture experiments of Penaeus vannamei, one Bacillus licheniformis ( BL-9), one Bacillus subtilis ( BS-12), and one Pseudoalteromonas flavipulchra ( CDM8) were selected to Make up a compound probiotics On a scale of 1 to 1 to 1.The experiments included a temporary period (7 days), a probiotics immersion period (15 days), and a Vibrio parahaemolyticus infection period (10 days).The experiment results showed that the addition of compound probiotics in the culture water could significantly increase the total number of culturable bacterias in the water and the intestinal tract of Penaeus vannamei (P < 0.05).The cumulative survival rate of shrimp in the experimental group was 73.3% at the end of the infection experiment. The cumulative survival rate of shrimp was significantly higher than that of the positive control group (25.3%).Immune-associated gene heat shock protein 70( Hsp70), β-1,3-glucan binding protein-lipoprotein (βGBP-HDL), Lipopolysaccharide-β-1, 3-glucan binding protein(LGBP), Lysozym(LZM),Crustin, Immune related enzymes catalase (CAT),LZM ,was up-regulated in different degrees in the immune phase, and expressed in different levels in Vibrio parahaemolyticus infection stage. The results suggested that the compound probiotics with Bacillus spp and Pseudomonas alternata in water could improve the ability of shrimp to resist Vibrio parahaemolyticus infection, and the increase of resistance ability of Shrimp might be antagonistic in bacterias, and the expression level of disease-related genes was related.
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