文章摘要
鞍带石斑鱼(♂)与褐石斑鱼(♀)、云纹石斑鱼(♀)杂交后代变态发育和生长比较
Comparison of Metamorphosis Development and Growth of Hybrids Offspring between Epinephelus lanceolatus (♂) and E.bruneus (♀) ,E. moara (♀)
投稿时间:2019-05-16  修订日期:2019-06-26
DOI:
中文关键词: 褐石斑鱼  云纹石斑鱼  鞍带石斑鱼  杂交子一代  仔稚幼鱼  形态发育
英文关键词: Epinephelus bruneus  Epinephelus moara  Epinephelus lanceolatus  Crossbreed F1  Larvae, juveniles, young fish  Morphological development
基金项目:山东省良种工程(2016LZGC009)、烟台市重点研发计划(2016JH021)、烟台市高端人才引进“双百计划”项目和黄海水产研究所基本科研业务费(20603022019002;20603022018019)
作者单位E-mail
吴玉萍 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛大连海洋大学水产与生命学院 大连 wuyuping12@foxmail.com 
田永胜 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛青岛海洋科学与技术试点国家实验室 海洋渔业科学与食物产出过程功能实验室 青岛 tianys@ysfri.ac.cn 
成美玲 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛大连海洋大学水产与生命学院 大连  
李振通 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛上海海洋大学水产与生命学院 上海  
张晶晶 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛上海海洋大学水产与生命学院 上海  
王林娜 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛青岛海洋科学与技术试点国家实验室 海洋渔业科学与食物产出过程功能实验室 青岛  
马文辉 莱州明波水产有限公司 莱州  
庞尊方 莱州明波水产有限公司 莱州  
张淞林 莱州明波水产有限公司 莱州  
翟介明 莱州明波水产有限公司 莱州  
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中文摘要:
      本文利用鞍带石斑鱼(Epinephelus lanceolatus)冷冻精子分别与褐石斑鱼(Epinephelus bruneus)、云纹石斑鱼(Epinephelus moara)卵进行人工授精,对两种杂交子代褐龙石斑鱼(E. bruneus♀×♂E. lanceolatus)和云龙石斑鱼(E. moara♀×♂E. lanceolatus)的变态发育、仔稚幼鱼的生长进行观察和比较。结果显示,两个杂交后代受精率相近,但褐龙石斑鱼畸形率高于云龙石斑鱼,孵化率低于云龙石斑鱼。根据卵黄囊的消长、第二背鳍棘和腹鳍棘长度变化、鳞片及体色的变化,杂交后代的胚后发育经历仔鱼期、稚鱼期和幼鱼期完成变态发育。在温度24-26 ℃,盐度29-31,DO ≥ 10 mg/L的条件下,褐龙石斑鱼与云龙石斑鱼生长发育至5 d卵黄囊消失;培育至41 d同时进入稚鱼期,此时第二背鳍棘、腹鳍棘长度分别为9.74 ± 0.42 mm、7.79 ± 0.39 mm和9.59 ± 0.46 mm、5.81 ± 0.09 mm,达到最长值,之后开始退化缩短;褐龙石斑鱼发育至51d稚鱼完成变态进入幼鱼期,全长25.46 ± 1.16 mm,云龙石斑鱼稍晚(56 d),此时全长23.24 ± 1.21 mm;62 d褐龙石斑鱼体表出现4条褐色斑带,云龙石斑鱼体表出现6条褐色斑带;生长至70 d时褐龙石斑鱼全长是云龙石斑鱼的1.04倍。褐龙石斑鱼全长日生长公式为L= 2.6208e0.0465x, R2 = 0.9603;云龙石斑鱼全长日生长式为 L=2.7158e0.0453x, R2 = 0.9451。褐龙石斑鱼肛前距与日龄关系式P= 1.3371e0.0483x, R2 = 0.9451, R2 = 0.9643;云龙石斑鱼肛前距与日龄的方程式为P=1.4017e0.0467x, R2 =0.944。褐龙石斑鱼与云龙石斑鱼全长日增长率均在1日龄时达到最大,分别为27.89%,30.03%;13d时褐龙石斑鱼肛前距日增长率达到最大(25.21%),云纹石斑鱼12d时达到最大(27.01%)。研究结果显示褐龙石斑鱼表现出较云龙石斑鱼生长更快的杂种优势,本文同时为同父异母石斑鱼杂交后代的生长及进一步育种提供了丰富的生物学数据。
英文摘要:
      Frozen sperm from Epinephelus lanceolatus with eggs from Epinephelus bruneus and Epinephelus moara for artificial insemination was performed, respectively.The metamorphosis development and growth of larval, juvenile of the two hybrid offspring, Helong grouper (E. bruneus♀×♂E. lanceolatus) and Yunlong grouper (E. moara♀×♂E. lanceolatus) ,were observed and compared. The results showed that the fertilized rate of the two hybrids was similar, but the malformation rate of the Helong grouper was higher than that of the Yunlong grouper, and the hatching rate was lower than that of the Yunlong grouper. According to the changes of the yolk sac, the length of the second dorsal fin spine and the first pelvic fin spine, and the changes of the scales and body color, the postembryonic development of hybrid offspring completed the metamorphosis development underwent the larval stage, the juvenile stage and the young stage. At the temperature of 24-26 ℃ , and salinity of 29-31, DO ≥10 mg/L, the yolk sac of Helong grouper and Yunlong grouper disappeared after 5 d; At 41 d, these two species of fish entered the juvenile stage at the same time , the lengths of the second dorsal fin spine and the pelvic fin spine were 9.74 ± 0.42 mm, 7.79 ± 0.39 mm and 9.59 ± 0.46 mm, 5.81 ± 0.09 mm, respectively, reaching the longest value, and then began to shorten. At 51days, juvenile fish of Helong grouper completed metamorphosis and entered the juvenile stage, with the total length of 25.46 ± 1.16mm , Yunlong grouper’s 56 d, is a little bit later, with the total length of 23.24 ± 1.21 mm. At 62 d, there were 4 brown patches on the body surface of Helong grouper, and 6 brown patches on the body surface of Yunlong grouper; The growth rate of Helong grouper was greater than that of Yunlong grouper, and the whole length of Helong grouper was 1.04 times as long as that of Yunlong grouper at 70d, it showed a faster growing heterosis than the Yunlong grouper. Daily growth formula of the full length of Helong grouper was L= 2.6208e0.0465x, R2 = 0.9603; Yunlong grouper’s was L=2.7158e0.0453x, R2 = 0.9451. The relationship between preanal distance and days of Helong grouper was P= 1.3371e0.0483x, R2 = 0.9643; Yunlong grouper’s wasP=1.4017e0.0467x, R2 =0.944. The daily growth rates of full length of Helong grouper and Yunlong grouper reached the maximum at 1 d, was 27.89% and 30.03%, respectively. At 13 d, daily growth rate of preanal distance of brown Helong grouper reached the maximum (25.21%), and that of Yunlong grouper reached the maximum (27.01%) at 12 d. The results showed that Helong grouper expressed faster heterosis than Yunlong grouper, and provided abundant biological data for the growth and further breeding of crossbred offspring of the same father but a different mother grouper in this paper.
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