文章摘要
江苏省4个太湖新银鱼种群遗传多样性和遗传结构分析
Genetic diversity and population structure of four Neosalanx taihuensis populations in Jiangsu province
投稿时间:2019-06-11  修订日期:2019-07-23
DOI:
中文关键词: 太湖新银鱼  细胞色素b基因  遗传多样性  遗传结构
英文关键词: Neosalanx taihuensis  Cyt b gene  genetic diversity  genetic structure
基金项目:
作者单位E-mail
李大命 江苏省淡水水产研究所资源与环境研究室 ldm8212@126.com 
唐晟凯 江苏省淡水水产研究所资源与环境研究室  
刘燕山 江苏省淡水水产研究所资源与环境研究室  
谷先坤 江苏省淡水水产研究所资源与环境研究室  
刘小维 江苏省淡水水产研究所资源与环境研究室  
殷稼雯 江苏省淡水水产研究所资源与环境研究室  
张彤晴 江苏省淡水水产研究所资源与环境研究室  
潘建林 江苏省淡水水产研究所资源与环境研究室 3186562149@qq.com 
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中文摘要:
      太湖新银鱼是我国特有的银鱼种类,主要分布在长江和淮河中下游及其附属湖泊,近年来其资源量呈明显下降趋势。为了解太湖新银鱼遗传背景,本研究采用线粒体细胞色素b(cytochrome b, Cyt b)基因序列,分析了江苏省太湖、高邮湖、洪泽湖和骆马湖4个太湖新银鱼野生群体共144尾样本的遗传多样性及遗传结构。结果表明:太湖新银鱼Cyt b基因序列共发现29个变异位点,定义25个单倍型;平均单倍型多样性为0.682±0.037,核苷酸多样性为0.00231±0.00021;4个群体中高邮湖群体的遗传多样性最高(Hd: 0.609±0.078, π: 0.00094± 0.00027),太湖群体的遗传多样性最低(Hd: 0.343± 0.107, π: 0.00075±0.00033)。分子方差分析(AMOVA)显示,太湖新银鱼群体间遗传差(71.53%)大于群体内(28.47%)遗传差异,遗传变异主要来自于群体间。遗传分化指数Fst值统计检验表明,骆马湖群体与太湖、高邮湖和洪泽湖群体之有显著性差异。分子系统树和单倍型网络进化图分析表明,25个单倍型形成2个明显地理分支,一支由太湖群体、高邮湖群体和洪泽湖群体组成,另一支由骆马湖群体组成。中性检验和错配分布图分析表明,太湖新银鱼历史上发生过群体扩张。整体来看,太湖新银鱼野生种群遗传多样性较低,应对其种质资源加强保护。建议将太湖群体、高邮湖群体和洪泽湖群体作为一个整体进行管理和保护,骆马湖群体单独进行管理和保护。
英文摘要:
      Neosalanx taihuensis is an endemic icefish in China, which mainly distribute in the middle and lower reaches of Yangtze River and Huaihe River and its affiliated lakes. In recent years, the natural resources of this species declined markedly. In order to assess genetic diversity and genetic structure of the wild populations of Neosalanx taihuensis, we amplified and sequenced mitochondrial Cytb gene sequences from 144 individuals of four lake wild N. taihuensis populations (Tai Lake(TH), Gaoyou Lake(GY), Hongze Lake(HZ) and Luoma Lake(LM)) in Jiangsu province. The results showed that 29 polymorphic nucleotide sites and 25 haplotypes were detected among the cyt b sequences of 144 individuals, the haplotype diversity was 0.682±0.037, and nucleotide diversity was 0.00231± 0.00021. The GY population showed the highest genetic diversity and TH population showed the lowest genetic diversity among the four populations. Analysis of molecular variance (AMOVA) showed that 71.53% molecular variation was among populations and 28.47% within populations, the molecular variation mainly orginated from interpopulation differences. The pairwise fixation index (Fst) revealed significant differences between LM population and TH, GY and HZ populations, and no significant differences among TH, GY and HZ populations. Phylogenetic tree and the minimum spanning network showed that the 25 haplotypes formed two distinct geographical clades: one clade consisted of TH, GY and HZ populations, and the other clade only consisted of LM population. The results of neutrality test showed that the values of Tajima’s D and Fu’s Fs were negative, and with significant difference, furtherly, the mismatch distribution was unimodal, which indicated that the four N.taihuensis populations had experienced population expansion in the history. In a word, the genetic diversity of N. taihuensis population was low, we should take more measures to protect its wild resources. Meanwhile, it is suggested that the TH, GY and HZ populations should be paid to protected and managed as a whole population and the LM population as a sole population.
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