文章摘要
条斑紫菜丝状体不同发育时期对光照和温度的光合适应能力
Effects of Light Intensity and Temperature on Photosynthetic Adaptability of the different stages of Pyropia yezoensis free-living conchocelis
投稿时间:2019-09-06  修订日期:2019-10-30
DOI:
中文关键词: 紫菜  营养藻丝  孢子囊枝  光合作用  高温  高光
英文关键词: Pyropia  Vegetative conchocelis  Conchosporangial branches  Photosynthesis  High light intensity  High temperature
基金项目:国家重点研发计划,山东省农业良种工程重大课题,
作者单位E-mail
魏家慧 中国水产科学研究院黄海水产研究所 997427166@qq.com 
李国梁 中国水产科学研究院黄海水产研究所  
汪文俊 中国水产科学研究院黄海水产研究所 wjwang@ysfri.ac.cn 
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中文摘要:
      以条斑紫菜(Pyropia yezoensis)丝状体为材料,研究了温度(15、25和35℃)与光强(40、100和300μmol·m-2·s-1)对营养藻丝和孢子囊枝光合生理的影响,以期揭示紫菜丝状体发育的光温调控机制,为建立高效精准紫菜育苗技术奠定基础。结果显示:1) 15℃和25℃实验组,营养藻丝和孢子囊枝的PSII原初光能转化效率(Fv/Fm)、总光合速率(Pg)和净光合速率(Pn)均随光强升高而降低。在300 μmol·m-2·s-1下,营养藻丝的Fv/Fm和Pg趋于零,Pn为负值。在25℃、40 μmol·m-2·s-1下,营养藻丝的呼吸耗氧速率(Rd)在实验周期内一直显著高于孢子囊枝;其他组则随胁迫时间延长,两者间Rd差距逐渐缩小。总体上,在相同条件下,所测孢子囊枝Fv/Fm 、Pg 和Pn均显著高于营养藻丝,而Rd与营养藻丝相当。2) 35℃实验组,6h时孢子囊枝的Fv/Fm显著高于营养藻丝,但随光强升高直线下降 (P<0.05),其他组Fv/Fm均趋于0。在40、100 μmol·m-2·s-1下,6h时孢子囊枝 Pg和Rd高于营养藻丝或两者相当;在300 μmol·m-2·s-1下,后期营养藻丝Pn和Rd高于孢子囊枝,但整个实验周期两者Pn全为负值。总体上,35℃下营养藻丝和孢子囊枝的Fv/Fm 、Pg 和Pn均显著低于(多数趋于0或负值)而Rd高于15℃和25℃下;后期,两种藻丝均出现发绿变白死亡现象。综上,在条斑紫菜营养藻丝的光合作用被严重抑制的光、温条件下,孢子囊枝仍具备相对高的光合活力,说明温度和光强升高到不利于营养藻丝生长的情况下,刺激藻丝转向了孢子囊枝发育阶段,后者具备更强的抗高温、高光胁迫能力。
英文摘要:
      The photosynthetic physiology of the different stages of Pyropia yezoensis free-living conchocelis was investigated under different temperatures (15, 25 and 35℃) and light intensities (40, 100 and 300 μmol·m-2·s-1 ), which was to reveal the regulation mechanism, and establish a foundation for efficient and accurate Pyropia yezoensis seedling technology. The main results were shown as follows: 1) At 15℃ and 25℃, the optimum PS II quantum yield (Fv/Fm), the gross photosynthesis rate (Pg) and the net photosynthetic rate (Pn) of the vegetative conchocelis and conchosporangial branches decreased as light intensity increased. Fv/Fm and Pg of vegetative conchocelis filament dropped to zero under 300 μmol·m-2·s-1, and the values of Pn were below zero. The respiratory oxygen consumption rate (Rd) of the vegetative conchocelis was significantly higher than that of the conchosporangial branched under 25℃, 40 μmol·m-2·s-1. The difference of the Rd values between vegetative and conchosporangial branches decreased gradually during culturing under the other conditions. In general, Fv/Fm, Pg and Pn of the conchosporangial branches were significantly higher than those of the vegetative conchocelis filament and there was no significant difference of Rd between the two developmental stages under the most culture conditions. 2) Fv/Fm of the conchosporangial branches was significantly higher than the vegetative conchocelis with 6h of culture under 35℃, which decreased with increasing light intensities. Fv/Fm of the other treatments was nearly 0; Pg and Rd of the conchosporangial branches were higher than or equivalent to the vegetative conchocelis with 6h culture under 35℃, 40 or 100μmol·m-2·s-1. After 1d or 2d, Pn and Rd of the vegetative conchocelis were higher than those of the conchosporangial branches while the Pn and Rd were all below zero under 300μmol·m-2·s-1 during the complete experimental period. On the whole, Fv/Fm, Pg and Pn of free-living Pyropia yezoensis conchocelis were significantly lower (mostly 0 or below zero) while the Rd was higher under 35℃ than under 15℃ and 25℃. After 1-3d, both vegetative conchocelis and conchosporangial branches became green or white. In summary, under the light intensity and temperature conditions in which the vegetative conchocelis was severely inhibited, conchosporangial branches has a relatively high photosynthetic activity. The present findings indicated that the high light intensity and temperature was not beneficial to vegetative conchocelis, but it can stimulate the vegetative conchocelis to turn to the conchosporangial branches, and conchosporangial branches has stronger resistance ability under high temperature and light intensities stress.
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