文章摘要
恢复时间对力竭训练后的斑节对虾幼虾的抗氧化功能的影响
The Effect of Recovery Time on Antioxidant Capacity of Juvenile Penaeus Monodon after Exhaustive Exercise
投稿时间:2019-09-10  修订日期:2019-10-11
DOI:
中文关键词: 斑节对虾  抗氧化能力  力竭运动  恢复过程
英文关键词: Giant tiger prawn,Oxidation resistance,Exhaustive exercise,Recovery process
基金项目:中国水产科学研究院基本科研业务费专项项目“海洋牧场应用技术研发及示范”(2016HY-ZD01);2019年农业资源及生态保护资金补助资金(增殖放流)项目“海洋增殖放流效果检验”(粤农农计〔2019〕3号)
作者单位E-mail
陈梓聪 中国水产科学研究院 南海水产研究所 462290205@qq.com 
陈丕茂 中国水产科学研究院 南海水产研究所 chenpm@scsfri.ac.cn 
袁华荣 中国水产科学研究院 南海水产研究所  
冯雪 中国水产科学研究院 南海水产研究所  
佟飞 中国水产科学研究院 南海水产研究所  
龙鑫玲 中国水产科学研究院 南海水产研究所  
陈文静 中国水产科学研究院 南海水产研究所  
张皓铭 中国水产科学研究院 南海水产研究所  
摘要点击次数: 282
全文下载次数: 0
中文摘要:
      力竭运动常出现于虾类养殖过程和野生环境中,斑节对虾(Penaeus monodon)是中国沿海重要养殖和增殖放流品种,但尚无关于斑节对虾力竭运动后生理恢复过程报道。该研究以斑节对虾幼虾为实验对象,在室内水槽使用抄网追逐斑节对虾幼虾,模拟迫使其进行力竭运动实验,探究其力竭运动后抗氧化能力恢复过程。结果显示,力竭运动后第0h,幼虾体内的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,总抗氧化能力(T-AOC)和丙二醛(MDA)含量,均与对照组无显著差异;第1~12h,SOD、CAT、POD活性、MDA含量和T-AOC持续下降,GSH-Px活性无显著变化;第24h,SOD、CAT、POD活性显著降低,GSH-Px活性、MDA含量、T-AOC显著升高;第72h,SOD、CAT活性仍显著低于对照组,GSH-Px、POD活性和MDA含量恢复至对照组,T-AOC显著高于对照组。在力竭运动过程中幼虾的酶促抗氧化系统未被激活;幼虾的抗氧化能力在力竭运动后的24h内较低,不宜再次受刺激;幼虾的抗氧化能力在力竭后24h后显著上升,并保持较长时间。
英文摘要:
      Exhaustive exercise often occurs in shrimp farming and in the wild. Giant tiger prawn (Penaeus monodon) is an important species of aquaculture and proliferation in China's coastal areas. The study used the dip net to chase the juvenile prawns in the indoor sink, simulated the exhaustive exercise experiment, and explored the recovery process of antioxidant capacity after exhaustive exercise, however, there was no report on the physiological recovery process after the exhaustive exercise of P. monodon. The results showed that the activities of superoxide dismutase(SOD), catalase(CAT), peroxidase(POD) and glutathione peroxidase(GSH-Px), total antioxidant capacity(T-AOC) and malondialdehyde(MDA) content in juvenile prawns at 0 h after exhaustive exercise No significant difference from the control group. At 1st~12thh, the activities of SOD, CAT, POD, MDA content and T-AOC continued to decrease, and the activity of GSH-Px did not change significantly. At 24thh, the activities of SOD, CAT and POD were significantly lower than those of the control group, and the activity of GSH-Px, MDA content and T-AOC were significantly higher than those of the control group. At 72ndh, the activities of SOD and CAT were still significantly lower than the control group. The activities of GSH-Px , POD and MDA content were restored to the control group, and T-AOC was still significantly higher than the control group. It indicated that the enzymatic antioxidant system of P. monodon was not activated during exhaustive exercise. At 24thh after exhaustive exercise, the antioxidant capacity at 72ndh was still at a high level. The juvenile prawns do not activate the enzymatic antioxidant system during exhaustive exercise. The antioxidant capacity of juvenile prawns is lower within 24 hours after exhaustive exercise, then increases significantly after 24 hours of exhaustion and remained for a long time. Within 24 hours after exercise, the juvenile prawns should be avoided stimulating.
相关附件:   矢量图  附件1
View Fulltext   查看/发表评论  下载PDF阅读器
关闭