文章摘要
基于SSR标记的刺参不同地理群体的遗传结构分析及指纹图谱构建
Genetic Structure Diversity Analysis and Fingerprint Construction of Different Geographical Populations of Sea Cucumber(Apostichopus japonicus) Based on SSR Markers
投稿时间:2019-11-25  修订日期:2019-12-19
DOI:
中文关键词: 刺参  SSR标记  遗传多样性  指纹图谱
英文关键词: Apostichopus japonicus  SSR markers  Genetic Diversity  Fingerprint
基金项目:国家重点研发计划课题(2018YFD0901603)
作者单位E-mail
廖梅杰 中国水产科学研究院黄海水产研究所 liaomj@ysfri.ac.cn 
王锦锦 中国水产科学研究院黄海水产研究所  
李 彬 中国水产科学研究院黄海水产研究所  
王印庚 中国水产科学研究院黄海水产研究所 wangyg@ysfri.ac.cn 
荣小军 中国水产科学研究院黄海水产研究所  
张 正 中国水产科学研究院黄海水产研究所  
范瑞用 青岛瑞滋集团有限公司  
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中文摘要:
      为分析中、韩、俄沿海刺参种质遗传结构,本研究采用SSR指纹图谱技术对中国青岛、烟台,韩国浦项、韩国群山、韩国木浦和俄罗斯符拉迪沃斯托克的8个不同地理种群的刺参群体进行遗传多样性分析和指纹图谱构建。结果显示13个微卫星座位的平均观察杂合度(H0)和平均期望杂合度(He)分别为0.47和0.80。13个位点的多态信息含量(PIC)范围为0.465(AJ06)~0.909(AJ09),除AJ06为中度多态性(0.25<PIC<0.50)外,其余12个位点均为高度多态性(PIC>0.50)。单个位点的等位基因数(A)从10(AJ06)到34(AJ07),平均等位基因数为19.38个。各位点的有效等位基因共83.85个,各位点有效等位基因数(Ne)的范围为1.72(AJ06)到11.81(AJ09),平均有效等位基因数为6.5。对各群体的遗传多样性分析结果表明,8个群体的PIC指数范围为0.6392(SK-MP-B)到0.7122(QD),说明相应群体均具有较高的遗传多样性。构建DNA指纹图谱可将所采集的8个群体区分开。利用STRUCTURE软件进行遗传结构分析结果表明8个刺参群体分配到3个自由交配群中,分群结果与UPGMA聚类分析结果相一致。UPGMA聚类分析图显示,中国青岛群体、烟台群体与韩国木浦黑参群体聚为一支,而俄罗斯刺参、韩国浦项黄参群体、韩国群山黑参群体和韩国浦项黑参群体聚为一支,而韩国浦项红参群体作为外群,单独聚为一支。刺参分群及聚类分析表明不同群体的刺参遗传结构及遗传分化情况不仅与地理位置相关,还与刺参体色具有一定的相关性,相关研究结果可为保护刺参种质资源和不同地理种群刺参的鉴别提供技术支撑。
英文摘要:
      In order to evaluate the genetic diversity of sea cucumber (Apostichopus japonicus) populations,13 SSR loci were used to amplify 8 different geographical populations of sea cucumber collected from China, Korea and Russia. The results showed that the average observed heterozygosity (H0) and average expected heterozygosity (He) of 13 microsatellite loci were 0.47 and 0.80, respectively. The polymorphism index content (PIC) of 13 loci ranged from 0.465 (AJ06) to 0.909 (AJ09). Except that AJ06 was moderately polymorphic (0.25 < PIC < 0.5), the other 12 loci were highly polymorphic (PIC > 0.5). The number of alleles (A) ranged from 10 (AJ06) to 34 (AJ07), with an average of 19.38. The total number of effective alleles was 83.85. The number of effective alleles (Ne) ranged from 1.72 (AJ06) to 11.81 (AJ09), and the average number was 6.5. The results of genetic diversity analysis showed that the PIC ranged from 0.6392 (SK-MP-B) to 0.7122 (QD), indicating that all populations had high genetic diversity. Construction of DNA fingerprints can distinguish all of the eight populations. The eight populations of sea cucumber were allocated to three free mating groups using STRUCTURE software, which were consistent with those of UPGMA cluster analysis. UPGMA cluster analysis showed that Qingdao population, Yantai population and Mokpo black population were clustered into one group, while Russion population, Posco yellow population, Kunsan black population and Posco black population were clustered into another group. Posco red population was located in the exception group, separately gathered into a seperate branch. Cluster analysis showed that the genetic structure and differentiation of different populations of sea cucumber were not only related to geographical location, but also has relation with body color. The results could provide the foundation for the genetic identification and genetic resource conservation of sea cucumber.
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