文章摘要
云龙石斑鱼胃排空特征和摄食消化特性研究
Characteristics of gastric evacuation and feeding digestion in “Yunlong” grouprs (Epinephelus lanceolatus ♂ × E. moara ♀)
投稿时间:2019-12-16  修订日期:2020-01-05
DOI:
中文关键词: 云龙石斑鱼  胃排空率  数学模型  皮质醇  葡萄糖  消化酶
英文关键词: “Yunlong” groupers (Epinephelus lanceolatus ♂ × E. moara ♀)  Gastric evacuation rate  Mathematical model  Cortisol  Glucose  DigestiveSenzyme
基金项目:山东省重大科技创新工程项目(2018SDKJ0303-4)和科技部国家重点研发计划-蓝色粮仓科技创新专项(2019YFD0900904)
作者单位E-mail
高云红 上海海洋大学水产与生命学院 上海 gaoyunhong2016@163.com 
景琦琦 青岛国家科学与技术试点国家实验室 青岛  
黄 滨 中国水产科学研究院黄海水产研究所  
关长涛 中国水产科学研究院黄海水产研究所  
张佳伟 莱州明波水产有限公司 莱州  
李文升 莱州明波水产有限公司 莱州  
翟介明 莱州明波水产有限公司 莱州  
贾玉东 中国水产科学研究院黄海水产研究所 jiayd@ysfri.ac.cn 
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中文摘要:
      为阐明养殖新品种云龙石斑鱼(Epinephelus lanceolatus ♂ × E. moara ♀)摄食胃排空特征和消化酶活性变化规律,本实验检测了体质量为680.35±39.84 g云龙石斑鱼摄食后胃内容物百分比,比较了线性模型、平方根模型、立方模型三种数学模型对胃排空曲线的拟合程度,分析了云龙石斑鱼摄食后血清中葡萄糖、皮质醇含量和肝脏消化酶活性变化,并对胃排空率与消化酶进行了相关性分析。结果发现,云龙石斑鱼摄食后,胃排空率呈典型的先慢后快再慢的消化类型。直线模型、平方根模型、立方模型都能够拟合云龙石斑鱼的胃排空数据,其中立方模型拟合效果最佳。由立方模型可知云龙石斑鱼胃80 %排空(食欲基本恢复)时间为9.5小时,100 %排空(食欲完全恢复)约14.8小时。血清中葡萄糖、皮质醇含量及肝脏中消化酶(淀粉酶、糜蛋白酶、脂肪酶)的活性在摄食胃排空过程中均呈先升高后降低的趋势。摄食后,血清中皮质醇、葡萄糖含量和肝脏内消化酶活力逐渐升高,0 h ~ 3 h上升速率较慢,3 h ~ 6 h内呈急剧上升的变化趋势,且在摄食后6 h血清中皮质醇、葡萄糖含量和肝脏内消化酶活力达到最高(P<0.05),之后呈显著下降趋势,且云龙石斑鱼瞬时排空速率与消化酶活性呈正相关(P<0.05)。综上,基于云龙石斑鱼胃排空特征和摄食消化特性,结合生产实践,在食欲基本恢复后开始投喂,投喂间隔为10 小时左右,每日投喂2 次,效果最佳。
英文摘要:
      In order to identify the characteristics of gastric evacuation and the changes of digestive enzymes activity of “Yunlong” groupers (Epinephelus lanceolatus ♂ × E. moara ♀). In the present study, the gastric evacuation rate, digestive enzyme activities in the liver and the intestine, serum glucose and cortisol in “Yunlong” groupers (weight: 680.35 ± 39.84 g) were evaluated. Meanwhile, the degree of fitting of the gastric emptying curve by the linear model, square root model and the cubic root model were analyzed. The results showed that the gastric evacuation rate of “Yunlong” grouper was a typical curve digestive pattern of slow-fast-slow digestion during gastric evacuation process. Three mathematical models all can fit gastric evacuation data of “Yunlong” groupers, while cubic model obtained the best result. According to the cubic model, the time of 80 % (appetite basic recovery) and 100 % (complete recovery of appetite) gastric emptying occurred at 9.5 hours and 14.8 hours in “Yunlong” groupers, respectively. The hepatic and intestinal digestive enzymes (amylase, chymotrypsin, lipase) activities significantly increased from 0 to 6 h, and then decreased from 9 to 24 h during gastric emptying in “Yunlong” groupers. The contents of serum glucose and cortisol manifest similar result to digestive enzymes activities during gastric emptying in “Yunlong” groupers. In addition, serum cortisol, glucose and digestive enzymes activity is slowly increased from 0 h to 3 h, then dramatically increased from 3 h to 6 h (P<0.05), the highest values obtained at 6 h and then significantly decreased until gastric evacuation completed (P<0.05). Meanwhile, gastric evacuation rate positively correlated with digestive enzyme activity during gastric evacuation in “Yunlong” grouper. In conclusion, these results indicate the optimal interval feeding is recommended to be fed at 10 h and twice per day based on the stomach emptying characteristics and feeding digestion characteristics of Yunlong” groupers (Epinephelus lanceolatus ♂ × E. moara ♀).
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