文章摘要
不同养殖密度对中国对虾生长和能量代谢的影响
Effects of different stocking densities on growth and Energy Metabolism in Fenneropenaeus chinensis
投稿时间:2020-03-26  修订日期:2020-05-18
DOI:
中文关键词: 中国对虾  密度  生长  能量代谢
英文关键词: Fenneropenaeus chinensis  Stocking density  growth  Substance metabolism
基金项目:国家虾蟹产业技术体系(CARS-48)、山东省泰山产业领军人才工程项目(LNJY2015002)、国家自然科学基金(31772842)和山东省重点研发计划项目(2019QYTPY022)共同资助
作者单位E-mail
张海恩 中国水产科学研究院黄海水产研究所 1311350726@qq.com 
何玉英 中国水产科学研究院黄海水产研究所 heyy@ysfri.ac.cn 
李健 中国水产科学研究院黄海水产研究所  
胡硕 中国水产科学研究院黄海水产研究所  
韩旭 上海海洋大学 黄海水产研究所  
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中文摘要:
      本研究采用网箱养殖的方法,通过室内28d养殖实验,在4个养殖密度G1(100尾/ m3)、G2(200尾/ m3)、G3(300尾/ m3)、G4(400尾/ m3)下,研究不同养殖密度对中国对虾生长、能量代谢和代谢酶活力的影响。研究结果表明,G2组的增重率、相对增长率和特定生长率均最高,与G1组差异不显著(P>0.05);G3和G4组的体重差异系数显著升高(P<0.05),其它生长指标和成活率均显著低于G1组(P<0.05),G4组与G1组达差异极显著(P<0.01)。随密度增加,G3和G4组可以显著提高血淋巴葡萄糖、乳酸、丙酮酸含量(P<0.05),显著降低甘油三酯含量(P<0.05);G2组四种指标含量与G1组差异不显著(P>0.05),而氨基酸含量在不同密度之间变化不显著(P>0.05),说明密度胁迫下,表明在高密度能使对能源的需求增加,中国对虾倾向性利用糖类脂类作为能源。另外,G3和G4组的己糖激酶、丙酮酸激酶、6-磷酸果糖激酶和乳酸脱氢酶活性显著升高(P<0.05),说明机体氧化代谢途径发生变化,分解代谢增强。琥珀酸脱氢酶活性显著降低(P<0.05),表明三羧酸循环活性的降低,进一步表明密度胁迫抑制了组织的有氧代谢。综上,G3和G4组密度显著影响了中国对虾的生长,增加了对碳水化合物和脂类利用,抑制了组织的有氧代谢,所以当中国对虾规格为4.5g时,其养殖密度100-200尾/m3为宜。
英文摘要:
      The effects of Fenneropenaeus chinensis on growth indices, energy Metabolism content and metabolic enzyme activity at four socking densities (G1 (100ind/m3), G2 (200ind/m3), G3 (300ind/m3), G4 (400ind/m3)) were studied in a 28-day indoor culture experiment by using the cage culture method. The results show that the weight gain rate, relative growth rate and specific growth rate of the G2 group were the highest, and were not significantly different from G1 group (P>0.05). The weight difference coefficient of the G3 and G4 groups increased significantly (P<0.05). The weight gain rate, relative growth rate, specific growth rate and survival rate were significantly lower than G1 group (P<0.05), and the G4 group were extremely significant with G1 group (P<0.01). In addition, the G3 and G4 groups could significantly increase the levels of hemolymph glucose, lactic acid, pyruvate (P<0.05) and significantly reduce the content of triglycerides (P<0.05).The glucose, lactic acid, pyruvate and triglyceride four contents of the G2 group there was no significant difference from G1 group (P>0.05). But the amino acid content did not change significantly between different densities (P>0.05). Indicating that the high-density stress can increase energy demand, and F. chinensis tend to use sugars and lipids as energy sources under the stress of density. The activities of hexokinase, pyruvate kinase, 6-phosphate fructokinase and lactate dehydrogenase in the G3 and G4 groups were significantly increased (P<0.05), indicating that the body's oxidative metabolism pathways have changed and catabolism has increased. The succinate dehydrogenation enzyme activity was significantly reduced (P<0.05), indicating a decrease in tricarboxylic acid cycle activity and further indicating that density stress inhibited aerobic metabolism of tissues. In summary, the G3 and G4 groups significantly affected the growth and increased the use of carbohydrates and lipids, and inhibited the aerobic metabolism of the tissues of F. chinensis. Therefore, it is recommended that 100-200 ind/m3 be the appropriate density when the size of F. chinensis is 4.5 g.
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