文章摘要
菲律宾蛤仔对急性海水酸化和重金属(Cu、Cd)胁迫的生理响应
Physiological response of Ruditapes philippinarum to acute stress of seawater acidification and heavy metals (Cu and Cd) incubation
投稿时间:2020-05-11  修订日期:2020-05-27
DOI:
中文关键词: 菲律宾蛤仔  重金属  酸化  心率  生理代谢
英文关键词: Ruditapes philippinarum  heavy metal  ocean acidification  heart rate  physiological metabolism
基金项目:
作者单位E-mail
张雯雯 上海海洋大学水产与生命学院 上海 tosyll@qq.com 
丁敬坤 上海海洋大学水产与生命学院 上海  
李加琦 中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东省渔业资源与生态环境重点实验室 青岛  
薛素燕 中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东省渔业资源与生态环境重点实验室 青岛  
蒋增杰 中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东省渔业资源与生态环境重点实验室 青岛  
方建光 中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东省渔业资源与生态环境重点实验室 青岛  
毛玉泽 中国水产科学研究院黄海水产研究所 农业农村部海洋渔业可持续发展重点实验室 山东省渔业资源与生态环境重点实验室 青岛 maoyz@ysfri.ac.cn 
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中文摘要:
      为研究海水酸化和重金属污染物对滩涂贝类的联合影响,采用静态急性毒性实验方法,以自然海水(pH 8.2)为对照组,研究了不同酸化条件下(pH 7.7和7.3),菲律宾蛤仔(Ruditapes philippinarum)耗氧率、滤水率和心率对重金属Cu (0,0.06,0.60mg/L)和Cd (0,0.03,0.30mg/L)胁迫的生理响应。实验结果表明,Cu胁迫显著影响蛤仔生理活动(P<0.05),在0.06mg/L Cu处理组中,蛤仔的耗氧率[0.39 mg/(g?h)]和滤水率[0.19 L/(g?h)]显著低于对照组(P<0.05),其心率在实验开始20min内由23.8 bpm下降至10.5 bpm。两种浓度的Cd胁迫对蛤仔耗氧率和滤水率没有显著影响,但在0.30mg/L Cd 胁迫下,蛤仔心率显著升高(P<0.05),由26.0 bpm (0 min)升至39.0 bpm (20min)。在实验设定的海水酸化范围,短期的pH下降对蛤仔的耗氧率、滤水率没有显著影响,但在pH 7.3海水中蛤仔心率出现短暂下降并快速恢复的现象,实验中未发现pH与Cu或Cd存在显著交互作用。本研究有助于丰富海水酸化与重金属毒理作用在海洋贝类中的研究内容,为预防滩涂潜在重金属污染风险及生物修复提供参考。
英文摘要:
      Intertidal bivalves experience broad fluctuations of abiotic factors including temperature, salinity and pH, which could affect their physiology and biochemistry especially when combine with anthropogenic pollution. To study the impact of heavy metal exposure on intertidal bivalves under ocean acidification conditions, We determined the interactive effects of decreased pH and heavy metals (Cu or Cd) addition on physiological functions (including oxygen consumption rate (OR), filtration rate (FR) and heart rate) of Ruditapes philippinarum. Both of the two concentration of Cu (0.06 and 0.60mg/L) addition suppressed OR, FR and heart rate (P<0.05) of clams. The heart rate of clams decreased from 23.8 bpm (Beats Per Minute) to 10.5 bpm after being exposed to 0.06 mg/L Cu for 20 minutes. No significant effect of Cd was found on OR and FR of clams, however clams incubated in 0.30mg/L Cd group had a significantly higher rate of heart rate (39.0 bpm) than control (25.2 bpm). Decreases pH (7.7 and7.3) had no effect on OR and FR, but the heart rate of clams in pH 7.3 group decreased at the beginning of incubation and recovered rapidly. There was no significant interaction between pH and heavy metal addition. Based on oxygen consumption, filtration and cardiac activity, the order of toxicity was Cu > Cd > pH. Heart rate was a more sensitive endpoint than OR and FR. Our study provided referential data for studying the response of intertidal bivalves to heavy metal and ocean acidification, which may be helpful in prevention and biological reparation of potential heavy metal pollution risk in coastal area.
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