文章摘要
黄海中国对虾环境DNA(eDNA)的垂直分布规律及其影响因素初探
A preliminary study on the vertical distribution of environmental DNA (eDNA)of FenneropenaeusSchinensis in Yellow Sea and its influencing factors
投稿时间:2020-05-25  修订日期:2020-06-09
DOI:
中文关键词: 环境DNA  中国对虾  分布规律  表层沉积物  生物量评估
英文关键词: Environmental DNA  Chinese shrimp  Surficial sediment  Biomass assessment
基金项目:国家自然科学基金项目(31872692)、山东省支持青岛海洋科学与技术试点国家实验室重大科技专项(2018SDKJ0501-1)和山东省泰山学者专项基金项目、中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2019GH16)共同资助[This work was supported by the National Natural Science Foundation of China (31872692), the National laboratory of Marine science and technology pilot project of Qingdao, shandong province (2018SDKJ0501-1), the TaiShan scholars project of shandong province, and the special fund of basic scientific research operating expenses of the central public welfare research institutes of China academy of fishery sciences (2019GH16)].
作者单位E-mail
钱瑭毅 大连海洋大学 389168510@qq.com 
王伟继 中国水产科学研究院黄海水产研究所  
李苗 中国水产科学研究院黄海水产研究所  
单秀娟 中国水产科学研究院黄海水产研究所 shanxj@ysfri.ac.cn 
金显仕 中国水产科学研究院黄海水产研究所  
摘要点击次数: 228
全文下载次数: 0
中文摘要:
      准确掌握物种分布和种群动态是渔业资源评估的基础。然而,对于某些种群规模小或生活史复杂的物种进行监测难度很大。近年来,环境DNA技术快速兴起,已被广泛应用于各类物种监测、生物多样性评估和生物量评价等领域。为了解冬季中国对虾 (Fenneropenaeus chinensis) 越冬洄游过程中的分布情况,2019年12月份在黄海中南部采集表、中、底三个水层水样,检测其中中国对虾的eDNA,并对表层沉积物进行室内实验。研究发现,第一,中国对虾环境DNA在自然水体中呈现特殊的垂直分布规律:表现为底层浓度高,表层浓度低,这一规律与中国对虾生活习性相关;第二,表层沉积物会在外力作用下再悬浮并向周围释放eDNA,对水体造成较大程度影响,本研究将采集到的表层沉积物分为三个实验组,三个实验组最大释放量分别为1624.06 copies/L、3453.34 copies/L以及1143.24 copies/L,沉积物对水体的影响持续一周左右。希望通过本研究对以后的采样设计有所帮助。
英文摘要:
      Accurate knowledge of species distribution and population dynamics is the basis of fishery resource assessment. However, it is difficult to monitor certain species with small populations or complex life histories. Recently, as a new monitoring technology, environmental DNA technology has been widely used in species monitoring, biodiversity assessment and biomass assessment. In order to understand the distribution of Chinese shrimp during its winter migration. In December 2019, we collected water samples of three water layers in the south-central yellow sea to test the environmental DNA of Chinese shrimp. In addition, laboratory experiments were carried out on the surficial sediments of the seabed. It was found that, first, the environmental DNA of Chinese shrimp showed a special vertical distribution rule in the natural water, which was characterized by high concentration in the bottom layer and low concentration in the surface layer. This rule is related to the life habits of Chinese shrimp. Second, the surficial sediments would re-suspend and release eDNA to the surrounding areas under the action of external forces, causing a large impact on the water. In this study, surficial sediments collected were divided into three experimental groups, the maximum release amounts of the three experimental groups were 1624.06 copies/L, 3453.34 copies/L and 1143.24 copies/L. The effect lasted for about a week. It is hoped that this study will be helpful to the sampling design in the future.
相关附件:   附件1  附件2  附件3  附件4  附件5  附件1  附件2  附件3  附件4  附件5
View Fulltext   查看/发表评论  下载PDF阅读器
关闭