文章摘要
不同生长时期红螯螯虾肌肉和肝胰腺内氨基酸和脂肪酸变化分析
Analysis of Amino Acid and Fatty Acid of Muscles and Hepatopancreas of Red Crayfish at Different Growth Stages
投稿时间:2020-06-02  修订日期:2020-06-29
DOI:
中文关键词: 红螯螯虾  生长时期  肌肉  肝胰腺  氨基酸  脂肪酸
英文关键词: red crawfish  different growth stages  muscle  hepatopancreas  amino acid  fatty acid
基金项目:
作者单位E-mail
秦高婵 浙江海洋大学 2745932861@qq.com 
陈红林 浙江省农业科学院 shmilyweight@126.com 
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中文摘要:
      为了研究不同生长时期红螯螯虾(Cherax quadricarinatus)肌肉和肝胰腺内脂肪酸和氨基酸的动态变化,选取90(第二性征未发育)、140(第二性征发育成熟)、180(性腺发育成熟)日龄的红螯螯虾,分别测定其肌肉和肝胰腺内氨基酸和脂肪酸的含量。结果显示:肌肉的16种氨基酸中,必需氨基酸总量90天时显著高于其他两个时期(P<0.05);而红螯螯虾肝胰腺内必需氨基酸总量和非必需氨基酸总量均随着日龄增加逐渐下降。比较不同生长时期肌肉中脂肪酸含量发现,随着日龄增长,饱和脂肪酸(SFA)含量呈下降趋势,而单不饱和脂肪酸(MUFA)含量呈先上升后下降的变化趋势,在140天时含量最高(25.69 ± 0.42 %),多不饱和脂肪酸(PUFA)含量呈上升的变化趋势;肝胰腺中SFA含量在三个生长时期内无显著性差异(P>0.05),MUFA含量呈先上升后下降的变化趋势,在140天时含量最高(37.44 ± 0.59 %),而PUFA含量变化与MUFA正好相反。营养价值评估结果显示,180日龄(可上市销售)的红螯螯虾肌肉内必需氨基酸指数(49.96 %)高于联合国粮农组织/世界卫生组织(FAO/WHO)评分模式(31.50 %)和全鸡蛋蛋白质模式(43.10 %),是较为优理想的优质食物蛋白源。由上可知,在不同生长时期,肌肉和肝胰腺中的氨基酸、脂肪酸的主要消耗不同,研究结果对于了解不同生长时期红螯螯虾营养需求,据此开发红螯螯虾配合饲料提供试验参考依据。
英文摘要:
      To investigate the changes of fatty acids and amino acids in muscle and hepatopancreas of red crayfish (Cherax quadricarinatus) at different growth periods, 90 (second sex is not developed), 140 (second sex is mature) and 180 (gonad matured) day-old red crayfish were selected for the experiment. The muscle and hepatopancreas were sampled from experimental red crayfish for chemical analysis. Results showed that 16 kinds of amino acids in muscle were determined and the content of essential amino acids (EAAs) at 90 days was significantly higher than the other two periods (P<0.05). However, the content of EAAs and non-essential amino acids (NEAAs) in the hepatopancreas of crayfish decreased gradually. As for fatty acid, the saturated fatty acids (SFA) in muscle showed a decreased trend; the content of monounsaturated fatty acid (MUFA) increased first and then decreased, which reached the highest content at 140 days (25.69 ± 0.42 %); the content of polyunsaturated fatty acid (PUFA) increased from 90 to 180 days of age in the red crayfish. While the SFA content in the hepatopancreas had no significant difference among the three growth periods (P>0.05); the MUFA content showed an increasing trend at first and then decreasing, which was highest at 140 days (37.44 ± 0.59 %); but the PUFA content showed the opposite trend of MUFA. Moreover, the nutritional value evaluation results showed that the essential amino acid index (49.96%) of muscle of 180-day-old (marketable) red crayfish was higher than the FAO/WHO score model (31.50%) and the whole egg protein model (43.10%), which was an ideal source of high-quality protein. From the above research results, we know that the main consumption of amino acids and fatty acids in muscle and hepatopancreas is different. These results provide a theoretical reference for understanding the nutritional requirements of red crawfish at different growth periods and developing corresponding feeds.
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