文章摘要
中间球海胆体腔细胞损失后的恢复规律及恢复期中轴器观察*
Recovering pattern of coelomocytes after lost in Strongylocentrotus intermedius and axial organ observation during the recovering phase
投稿时间:2020-07-08  修订日期:2020-08-13
DOI:
中文关键词: 中间球海胆  体腔细胞  恢复规律  中轴器  造血组织
英文关键词: Strongylocentrotus intermedius  Coelomocyte  Recovering pattern  Axial organ  Hemopoietic tissue
基金项目:
作者单位E-mail
纪锦霖 大连海洋大学盐城工学院 840048695@qq.com 
孟少东 大连海洋大学盐城工学院  
王轶南 大连海洋大学盐城工学院 16693762@qq.com 
丁君 大连海洋大学  
李强 盐城工学院,水生动物免疫与疾病研究所,盐城 江苏  
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中文摘要:
      海胆的体腔细胞在机体免疫防御中承担重要角色,探明其修复规律与来源可为深入研究海胆的免疫机制提供依据。本研究通过人工抽离中间球海胆(Strongylocentrotus intermedius)体质量10%的体腔液诱导体腔细胞的修复更新,分组测定6h、12h、18h及24h后海胆围脏腔内的体腔细胞密度,分析体腔细胞的修复规律;同时,取各时间点海胆的中轴器进行组织学观察,并利用鼠抗人Ki-67(细胞增殖抗原)单克隆抗体检测中轴器内的细胞增殖信号。结果显示,人工抽离体腔液后6h,体腔细胞密度与初始密度相比明显偏低;12h后逐步升高,密度略低于初始密度,恢复度为(92.78±29.40)%;18h后已明显高于初始密度,恢复度为(137.08±32.40)%,其显著高于6h及12h恢复度(P<0.05),随后体腔细胞密度逐渐降低。海胆损失体腔液后中轴器表现为内部组织消减、空腔化,外壁上皮细胞层崩解、脱离,凸起结构空泡化等变化,18h后内部疏松组织略有增加,24h后中央腔内出现明显新生组织。利用Ki-67单抗检测发现正常海胆中轴器内具有一定的细胞增殖信号,该信号在损失体腔细胞18h后明显增强。结果表明,损失体腔细胞后,中间球海胆可启动快速恢复机制,而海胆的中轴器会发生明显的组织结构变化,组织内的细胞增殖活动也明显增强。研究结果为海胆体腔细胞的造血组织与发生机制研究提供了科学依据。
英文摘要:
      Recovering pattern of coelomocytes after lost in Strongylocentrotus intermedius and the axial organ during the recovering phase was Similar as heamocyte in other specicies, coelomocyte of sea urchin play important role in its immune system. Verifing of it’s recovering pattern and orgin would be vital to understand immune mechanism. Oxial organ is a glandular organ that has been supposed as hemopoietic tissue of sea urchin. Using monoclonal antibody anainst coelomocytes we had proved in previous study that the axial organ could serve at least as an storage tissue of coelomocyte. To provide more clues, recovering pattern of coelomocytes in sea urchin Strongylocentrotus intermedius was investigated as well as the changes of axial organ during recovering phase in the present paper. Coelomocyte density was examined at 6h, 12h, 18h, 24h post mannual extraction 10% body weight of coelomic fluid. Meanwhile, histology of the axial organ was observed, and cell proliferation acivity in it was detected with monoclonal antibody against Ki-67 (cell proliferation antigen). The results showed that the average coelomocytes density at 6h was obviously lower than that before extraction. Then it inreased gradually at 12h with density slightly lower than initial level and recovering value of (92.78±29.40)%. It was obviously higher than initial level at 18h, with recovering value of (137.08±32.40)%, which was significantly higher than those of 6h and 12h (P<0.05). After then, the coelomocytes density decreased gradually. The inter tissue of axial organ declined and cavitation occured after coelomocytes lost. The outer epithelial layer?became loose and broke off, and vacuolation was observed in the bulges of epithelial layer. The inter tissue increased at 18h, and newborn tissue appeared obviously at 24h. Using antibody of Ki-67, we found cell production signal in the axial organ from normal sea urchin and that signal enhanced obviously after 18h. The results indicated that restoring mechanism of coelomocyte could initiate rapidly after ceolomocytes lost, meanwhile histology changes as well as cell production activity enhancement might occur in the axial organ. The paper provide valuable references in further research on the orgin and ontogenesis of coelomocyte.
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