文章摘要
蜡样芽孢杆菌YB1对大菱鲆幼鱼生长性能、肠道消化酶、肝脏抗氧化酶及肠道组织结构的影响[*]
Effects of Bacillus cereus YB1 on growth performance, intestinal digestive enzymes, liver antioxidant enzymes and intestinal tissue structure of juvenile turbot (Scophthalmus maximus)
投稿时间:2020-07-10  修订日期:2020-08-28
DOI:
中文关键词: 蜡样芽孢杆菌  大菱鲆  酶活力  肠道组织结构  促生长
英文关键词: Bacillus cereus  Scophthalmus maximus  enzymatic activity  intestinal tissue structure  growth promotion
基金项目:现代农业产业技术体系专项(CARS-47-Z14); 烟台市科技创新发展计划项目(2020MSGY073)
作者单位E-mail
崔广鑫 上海海洋大学 水产科学国家级实验教学示范中心山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室 xin1gc@163.com 
孙娜 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室上海海洋大学 水产科学国家级实验教学示范中心  
王腾腾 山东省海洋资源与环境研究院  山东省海洋生态修复重点实验室  
陈钰臻 上海海洋大学 水产科学国家级实验教学示范中心山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室  
韩慧宗 山东省海洋资源与环境研究院  山东省海洋生态修复重点实验室 hanhuizong729@163.com 
姜海滨 山东省海洋资源与环境研究院  山东省海洋生态修复重点实验室  
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中文摘要:
      本研究旨在探究饲料中添加蜡样芽胞杆菌(Bacillus cereus)YB1对大菱鲆(Scophthalmus maximus)幼鱼生长性能、肠道消化酶活力和肝脏抗氧化酶活力以及肠道组织结构的影响。选取720尾初始体重为(3.6?0.7)g的大菱鲆幼鱼为研究对象,随机分为4组,每组3个平行,每个平行60尾。4组大菱鲆幼鱼在投喂的饲料中分别添加活菌量为0CFU/g(对照)、105CFU/g、106CFU/g、107CFU/g的YB1,养殖期为50d。结果显示:1)YB1添加量为107CFU/g时,大菱鲆幼鱼的增重率(WGR)和特定生长率(SGR)显著高于对照组(P<0.05)。2)YB1添加量为106CFU/g组的大菱鲆幼鱼肠道蛋白酶和淀粉酶活力分别提高了57.86%和82.37%,显著高于对照组(P<0.05);YB1添加量提高到107CFU/g时,脂肪酶的含量显著高于对照组(P<0.05)。3)饲料中添加YB1对大菱鲆幼鱼肝脏过氧化氢酶活力无显著性影响(P>0.05);YB1添加量为106CFU/g组的幼鱼肝脏丙二醛含量下降了42.03%,显著低于对照组(P<0.05);实验组大菱鲆幼鱼肝脏SOD酶活力相较于对照组有上升的趋势,差异不显著(P>0.05)4)YB1添加量为106CFU/g组大菱鲆幼鱼肠道肌层厚度增加最为显著(P<0.05),相较于对照组在实验末期提高了68.91%。研究表明,饲料中添加适量蜡样芽胞杆菌YB1具有促进大菱鲆幼鱼生长、提高幼鱼肠道消化酶和肝脏抗氧化酶活力以及改善幼鱼肠道组织结构的作用,推荐添加量为106CFU/g。
英文摘要:
      The turbot (Scophthalmus maximus) has been important economic marine fish species of industrial farming in northern China. With the unceasingly amplication of farming scale and great improvement of intensivism in recent years, the turbot farming diseases were frequent. Bacillus cereus YB1 would be used as a candidate strain of probiotics, which had animal safety for turbot with a certain concentration. The present study aimed to investigate the effect of bacterium Bacillus cereus (YB1) on growth performance, liver immune enzyme activity, intestinal digestive enzyme activity and intestinal tissue structure in juvenile turbot (S. maximus), which provided new probiotic strains for turbot aquaculture. A total of 720 healthy juvenile with an average of body (3.6?0.7) g were randomly divided into 4 groups with 3 replicates per group and 60 fish per replicate. The fish in the 4 group were fed diets containing YB1 at viable count of 0CFU/g (control), 105CFU/g, 106CFU/g, 107CFU/g at (21±2)℃, respectively. The experiment lasted for 50 days. The results showed that: the weight gain rate (WGR) and specific growth rate (SGR) were significantly higher in juvenile turbot fed by diet supplemented with 107CFU/g of YB1 than that of the fish fed by control diet (P<0.05). The amount of protease and amylase increased by 57.86% and 82.37% respectively in 106CFU/g group, which was significantly higher than that in the control group (P<0.05); The lipase content was significantly higher than that of the control group (P<0.05), When YB1 was added to 107CFU/g. The catalase activities in the fish fed by diet supplemented with YB1 was non-significant effect with control group. The malondialdehyde content of the juveniles fed with 106CFU/g YB1 dietary supplement were decreased by 42%, which was extremely lower than that in the control group (P<0.05); The SOD activity of the experimental group increased compared with the control group, but the difference was not significant (P>0.05). YB1 led to obviously increase the height and number of folds in turbot’s intestinal mucosa (P<0.05). Intestinal myometrium thickness was obviously increased in the 106CFU/g YB1 dietary supplement group than that of the control, which was 68.91% higher than that of the control group at the end of the experiment. There were few research about the effect bacterium Bacillus cereus (YB1) on growth performance, antioxidant capacity, intestinal tissue structure in juvenile turbot,in this study,suitable Bacillus cereus (YB1) could promote the growth of juvenile turbot, improve the activities of digestive enzymes and liver immune enzymes in intestine, improve the structure of intestine. This study shows that Bacillus cereus (YB1) can be used in turbot aquaculture, the recommended dosage is 106CFU/g.
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