文章摘要
菲在厚壳贻贝体内的富集与释放及其对HSP70 mRNA表达的影响
Enrichment and release of phenanthrene in Mytilus coruscus and its effect on HSP70 mRNA expression
投稿时间:2020-08-11  修订日期:2020-09-07
DOI:
中文关键词:   厚壳贻贝  生物富集  HSP70
英文关键词: Phenanthrene  Mytilus coruscus  Bioconcentration  HSP70
基金项目:国家重点研发科技计划项目“2017YFC1600705”,中央公益科研机构基础研究基金 (No. 2020TD14),国家贝类产业体系建设项目(CARS-49)
作者单位E-mail
曾梦妮 上海海洋大学 2731238959@qq.com 
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中文摘要:
      以厚壳贻贝为研究对象,开展不同暴露浓度(4 μg/L 、20 μg/L、100 μg/L)的菲(phenanthrene, PHE)的富集(10 d)和释放(5 d)试验,分别利用高效液相色谱法和荧光定量qPCR法分析了厚壳贻贝内脏团、外套膜、闭壳肌对PHE的富集和释放情况及HSP70基因表达量变化情况。结果表明:在10 d的富集阶段,厚壳贻贝3个组织对PHE的富集能力大小表现为内脏团>外套膜>闭壳肌;3个组织对PHE的富集含量随时间的增加而增加,同时也随着浓度的增加而增加;在释放试验阶段,厚壳贻贝3个组织中PHE含量在释放前期迅速下降,但在15 d时3个组织中PHE残留量仍均高于对照组;PHE对厚壳贻贝体内HSP70 m RNA诱导表达具有组织特异性,其中外套膜中HSP70表达量最高。研究结果为PAHs在贝类体内的富集动力学及致毒机制研究提供参考依据。
英文摘要:
      Phenanthrene(PHE) is one of the most abundant polycyclic aromatic hydrocarbons (PAHs) in aquatic ecosystem. It has strong hydrophobicity and is often adsorbed on the sediment and suspended particles in water, which has potential toxicity to aquatic organisms. Mussels (Mytilus coruscus) are widely distributed in the ocean and live in a fixed environment. They are filter feeders and have strong accumulation of organic pollutants in water. They are often used as indicator organisms for monitoring marine environmental pollution. In this study, the enrichment (10 d) and release (5 d) tests of PHE with different exposure concentrations were carried out in this study. Four groups of PHE (4 μ g / L, 20 μ g / L, 100 μ g / L) and artificial seawater control group (0.01% acetone) were set up. Three parallel experimental groups were set up in each group, and 16 mussels were placed in each group. Two mussels were randomly selected on 1th , 3th, 6th, 10th, 12th and 15th day. The visceral mass, outer membrane and closed shell muscle tissue were separated and stored at - 80 ℃ for detection and analysis. The enrichment and release of PHE and the change of HSP70 gene expression in visceral mass, outer membrane and closed shell muscle tissue were analyzed by HPLC and qPCR respectively. The results showed that the concentration of PHE in three tissues of mussels was visceral mass > outer membrane > closed shell muscle at the same time and concentration. At the same time, the enrichment content of PHE in the three tissues increased with the increase of time and concentration. This is due to the higher n-octanol / water partition coefficient and fat solubility of PHE, the filter feeding life of mussel itself, and the fact that tissues with high fat content are more likely to enrich PHE. In the release test stage, the PHE content in the three tissues of mussels decreased rapidly in the early stage of release, however, the residual amount of PHE in the three tissues was still higher than that in the control group on the 15th day. This is because the release of PHE in different tissues was mainly controlled by diffusion driven by thermodynamics, metabolic activity regulated by enzyme system and excretion when mussel was in the state of water release. And the results showed that the content of PHE in tissues of mussels began to decrease rapidly in the early stage of release, and gradually decreased with the extension of release time. The other results showed that HSP70 was induced to enhance the anti stimulation and survival ability of the organism under the stress of PHE. The induced expression of HSP70 mRNA in mussels was tissue-specific, and the expression level of HSP70 in outer membrane was the highest. The results provide a reference for the study of enrichment kinetics and toxic mechanism of PHE in shellfish.
相关附件:   附件1  万方20200811(6.38%)  万方20200813(5.43%)  附件1  附件2
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