文章摘要
凡纳滨对虾(Litopenaeusvannamei)不同生长阶段肠道内容物中细菌耐药性研究
Study on bacterial resistance in Litopenaeus vannameiIntestinal contents at different growth stages
投稿时间:2020-12-09  修订日期:2021-01-27
DOI:
中文关键词: 凡纳滨对虾(Litopenaeus vannamei)  细菌耐药性  抗生素耐药基因(ARGs)  qRT-PCR
英文关键词: Litopenaeus vannamei  bacterial antibiotic resistance  antibiotic resistance genes (ARGs)  qRT-PCR
基金项目:江苏省第十六批“六大人才高峰”高层次人才项目(NY-106)、江苏省农业科技自主创新资金-农产品产业发展关键技术创新
作者单位E-mail
杨泽禹 江苏省海洋水产研究所 779287258@qq.comqq.com 
万夕和 江苏省海洋水产研究所 E-mail: wxh1708@163.com 
史文军 江苏省海洋水产研究所  
王李宝 江苏省海洋水产研究所  
黎慧 江苏省海洋水产研究所  
沈辉 江苏省海洋水产研究所  
乔毅 江苏省海洋水产研究所  
蒋葛 江苏省海洋水产研究所  
成婕 江苏省海洋水产研究所  
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中文摘要:
      随着国家用药减量技术的推广,江苏地区凡纳滨对虾养殖过程多使用微生态制剂来调节水质,为避免对有益细菌生长的抑制,养殖过程中几乎不用抗生素。为了对其进行验证,本文选取江苏地区四种主要养殖模式凡纳滨对虾成虾和虾苗作为研究对象,利用K-B纸片法和qRT-PCR技术,研究了样本肠道内容物中可培养优势菌对四环素等12种抗生素的耐药性和tetA等9种抗生素耐药基因(ARGs)的丰度。结果显示,不同养殖模式中凡纳滨对虾肠道可培养细菌优势属为弧菌属;成虾肠道内可培养细菌种类和数量较虾苗显著上升(P<0.05),ARGs丰度显著下降(P<0.05);49株可培养优势菌对12种抗生素的耐药率均较低,7种抗生素的耐药菌占比低于5%,其中3种无耐药菌,占比最高仅为14.29%;不同养殖模式之间未表现出显著变化规律。研究结果提示,凡纳滨对虾育苗期可能会施用少量抗生素,江苏地区对虾成虾肠道菌群的耐药性状况好于前述苗期样品,耐药性研究结果验证了成虾养殖过程中较少使用抗生素的调查结果。
英文摘要:
      Litopenaeus vannamei culturing industry is an important part of rural economy in coastal areas. With the development of shrimp farming industry, diseases become occurring frequently. Aquatic animals have a low absorption rate of antibiotics. Excess antibiotics would remain in environment, just as water and soil, which can induce for bacterial antibiotic resistance and antibiotic resistance genes (ARGs). And then threat to human health and safety. Therefore, the evaluation of bacterial antibiotic resistance phenotype and antibiotic ARGs abundance can help to understand antibiotic pollution in a certain area. According to our preliminary investigation, more microecologics were used during Litopenaeus vannamei culturing in Jiangsu with the popularization of the national drug-reduction technology. To verify the above research results, four main culture models of Litopenaeus vannamei in Jiangsu were selected in this article as the research objects to explore contamination of antibiotic resistance bacteria(ARB) and ARGs in shrimp intestinal contents. Drug resistanceto12 antibiotics (tetracycline, doxycycline, fluorobenicol, chloramphenicol, sulfamethoxazole, cotrimoxazole, neomycin, gentamicin, ciprofloxacin, norfloxacin, ceftriaxone, cefotaxime) and the abundance of 9 ARGs (tetA, tetG, qnrA, qnrS, cmle3, floR, aadA, sul1, sul2) of culturable dominant bacteria in the intestinal contents of samples were studied using K-B disc diffusion method and qRT-PCR. The results showed that Vibrio was the dominant bacterial genus in the intestinal contents of Litopenaeus vannamei in different aquaculture models. The species and quantity of culturable bacteria in adult shrimp intestines increased significantly than postlarvae, while the abundance of ARGs declined significantly (P<0.05). The ARB proportion of tetracycline, doxycycline, sulfamethoxazole and cotrimoxazole are higher, which accounted for 14.29%, 12.24%, 10.20% and 10.20%, respectively. There were no significant changes observed among different aquaculture models. The results of this study indicated that t a small amount of antibiotics might be applied during the nursery stage of Litopenaeus vannamei in Jiangsu province, while less antibiotics were applied during the cultivation stage. The pollution level of bacterial resistance in adult Litopenaeus vannamei intestinal contents was lower than that in postlarvae which corroborate previous findings. In the subsequent research, the differences of bacterial antibiotic resistance and ARGs abundance in the intestinal contents of Litopenaeus vannamei under different aquaculture models can be further explored by combining the methods of metagenomics.
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