文章摘要
基于形态学和高通量测序的海水池塘生态养殖系统中浮游植物多样性比较
Comparison of phytoplankton community diversity in the ecological aquaculture system in marine pond revealed by morphological analysis and high-throughput sequencing
投稿时间:2020-12-29  修订日期:2021-02-02
DOI:
中文关键词: 生态养殖  浮游植物  多样性  形态学分析  高通量测序
英文关键词: Ecological aquaculture  Phytoplankton community  Diversity  Morphological analysis  High-throughput sequencing
基金项目:
作者单位E-mail
乔玲 浙江省海洋水产研究所 qiaoling1990123@126.com 
常志强 中国水产科学研究院黄海水产研究所  
李健 中国水产科学研究院黄海水产研究所 lijian@ysfri.ac.cn 
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中文摘要:
      利用形态学鉴定方法结合高通量测序技术,对2018年12月海水池塘生态养殖系统中浮游植物群落组成及多样性进行研究,结果表明,两种方法共鉴定浮游植物8个门,绿藻门和隐藻门为主要优势类群;共鉴定浮游植物39个属,仅有5个属包括裸藻属、颤藻属、拟菱形藻属、塔胞藻属和全沟藻属是两种方法鉴定结果中共有的;一些微微型(≤2 μm)和较小的微型浮游植物(>2 μm且≤10 μm)仅在高通量测序结果中发现,其中微微型浮游植物占了总浮游植物序列丰度的25.24%,较小的微型浮游植物占了总浮游植物序列丰度的60.42%;形态学方法调查的浮游植物多样性指数低于高通量测序方法。因此,利用形态学鉴定方法结合高通量测序技术,更能全面、准确的了解养殖生态系统中浮游植物群落结构及多样性。
英文摘要:
      With increasing concern over the negative environmental impact of mariculture, the ecological aquaculture based on multi-trophic has received extensive attention in recent years. Phytoplankton is an important component of aquaculture ecosystem. They are useful in maintaining water quality by uptake of nutrients during photosynthesis, and serve as a direct or indirect food source for cultured organisms. Some phytoplankton, such as diatoms and green algae is conducive to the growth of shrimp, crab, shellfish and fish, while some phytoplankton (such as cyanophytes and dinoflagellates) might be toxic to aquatic organisms in the aquaculture system. To comprehensively assess phytoplankton community composition and diversity in the ecological aquaculture system in marine pond in December 2018, a combination of the traditional morphological analysis and high-throughput sequencing were used in the present study. A total of 8 phyla were detected by both methods. Chlorophyta and Cryptophyta were the dominant groups. It is notable that phyla Haptophyta and Chrysophyta were only found by the high-throughput sequencing A total of 39 genera were detected by both methods, but only 5 genera including Eutreptiella, Oscillatoria, Pseudo-nitzschia, Pyramimonas and Teleaulax were consistent. Some picophytoplankton (≤ 2 μm) and small nanophytoplankton (> 2 μm and ≤ 10 μm) were detected by high-throughput sequencing but not observed by morphological analysis. Picophytoplankton represented 25.24% of total phytoplankton sequence abundance, and small nanophytoplankton accounted for 60.42% of total phytoplankton. Phytoplankton diversity revealed by morphological analysis was lower than that revealed by high-throughput sequencing. Therefore, the combination of the traditional morphological analysis and high-throughput sequencing will be useful to get a comprehensive understanding of phytoplankton community composition and diversity in aquatic ecosystems. This study is useful for the future research as a foundation study towards characterization of phytoplankton community dynamics in the ecological aquaculture system in marine pond.
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