文章摘要
鲤trim25多拷贝基因进化和表达调控初探
Gene Evolution and Expression Regulation of Multiple Copies of Trim25 in Common Carp
投稿时间:2021-01-27  修订日期:2021-03-01
DOI:
中文关键词:   TRIM25  蛋白结构  系统进化树  eQTL
英文关键词: Cyprinus carpio  TRIM25  protein structure  phylogenetic tree  eQTL
基金项目:
作者单位邮编
朱优秀 上海海洋大学 201306
江炎亮 中国水产科学研究院 
张芹 河南省水产科学研究院
河南省水产科学研究院 
冯建新 河南省水产科学研究院
河南省水产科学研究院 
张瀚元 中国水产科学研究院 
吴碧银 上海海洋大学 
许建① 中国水产科学研究院 100141
摘要点击次数: 42
全文下载次数: 0
中文摘要:
      天然免疫系统是硬骨鱼类抵抗病毒感染的主要防御系统,TRIM蛋白家族作为天然免疫系统的重要组成部分,参与了病毒感染的免疫网络调控,其中TRIM25已被证实在多种鱼类的免疫反应中发挥重要作用。本研究对鲤trim25基因的16个拷贝进行了序列进化分析、共线性分析和功能域结构分析,并比较了各拷贝在组织中的表达和顺式调控位点的差异。序列比对和系统进化分析结果均表明,鲤与金线鲃和斑马鱼的TRIM25蛋白结构高度相似,与鲤科鱼类以外的其它物种的结构差异较大。基因共线性结果显示,trim25基因上下游基因在不同物种的进化过程中相对保守。鲤TRIM25蛋白的结构分析表明,在鲤TRIM25的16个拷贝中,有6个具有完整功能结构域的拷贝,其中各有5个拷贝在鲤的肝与脑组织中高表达。在构建的eQTL调控网络中,在肝和脑组织中分别筛选到5个和17个顺式调控trim25基因表达的SNP位点。本研究对鲤trim25基因多个拷贝的序列差异进行了比较,并对鲤与其他物种TRIM25的序列、进化关系和共线性相似度进行了比较,揭示了鲤trim25各拷贝间的结构多样性和在组织中的表达情况,筛选出了可能调控trim25基因表达的SNP位点,为今后研究鲤TRIM25相关的调控和抗病研究提供了理论依据。
英文摘要:
      The innate immune system is the main defense system against virus infection in teleost fish. As an important part of the innate immune system, the TRIM protein family participates in the regulation of immune network during virus infection, among which TRIM25 has been reported to play an important role in the immune response in many types of fish. In this study, 16 copies of the trim25 gene in common carp (Cyprinus carpio) were compared with other species by phylogenetic and syntenic analysis. Functional domain structures for 16 copies of TRIM25 protein were predicted, and the expression and cis-regulatory network of each gene copy in tissues were compared. Sequence alignment and phylogenetic analysis showed that the TRIM25 protein structure of Cyprinus carpio was highly similar to that of Spinibarbus auratus and Danio rerio, but distinct from that of other species beyond the Cyprinidae species. The results of gene synteny indicated that the upstream and downstream genes of the trim25 gene were relatively conservative in the evolution of different species. The structural analysis of TRIM25 protein showed that 6 copies had complete functional domains among the 16 copies of common carp, of which 5 copies were highly expressed in the liver and brain tissues, respectively. In the eQTL regulatory network for trim25, 5 and 17 SNPs were identified regulating trim25 expression in liver and brain tissues, respectively. In this study, the sequence differences of multiple copies of the trim25 gene in common carp were compared, and the evolutionary relationship and synteny of the trim25 gene were identified. The diversity of the structure and tissue expression of trim25 genes in the common carp were revealed, and the SNP sites that may regulate trim25 gene expression were screened out, providing reference for the future research on trim25 related regulation and disease resistance in the common carp.
相关附件:   附件1  附件1
View Fulltext   查看/发表评论  下载PDF阅读器
关闭