文章摘要
池塘养殖牙鲆肠道和环境菌群结构对益生菌制剂的响应
Responses of microbiota structure in intestinal tract and environment of pond culture Japanese flounder (Paralichthys olivaceus) to probiotics
投稿时间:2021-02-24  修订日期:2021-04-02
DOI:
中文关键词: 高通量测序  肠道菌群  牙鲆  池塘养殖  益生菌制剂
英文关键词: High throughput sequencing  Intestinal tract microbiota  Paralichthys olivaceus  Pond culture  Probiotcs
基金项目:
作者单位邮编
姜燕 中国水产科学研究院黄海水产研究所 266071
李存玉 沂南县水利局 
徐永江 中国水产科学研究院黄海水产研究所 
柳学周① 中国水产科学研究院黄海水产研究所 266071
于超勇 山东省海洋生物研究院 
王滨 中国水产科学研究院黄海水产研究所 
郑伟 山东寰达生态环境科技有限公司 
史宝 中国水产科学研究院黄海水产研究所 
摘要点击次数: 27
全文下载次数: 0
中文摘要:
      为了研究益生菌制剂对池塘养殖牙鲆肠道及环境菌群结构的调控效果,采用高通量测序技术和生物信息学分析手段构建了牙鲆肠道、养殖水体、饵料和池塘底泥的16S rDNA基因测序文库,分析了不同样品中菌群组成和多样性在益生菌制剂调控过程中的变化趋势。结果表明:添加益生菌制剂后,池塘底泥和牙鲆肠道的菌群多样性升高,且池塘底泥的依然是最高的;而养殖水体的菌群多样性明显下降,并低于牙鲆肠道的。牙鲆肠道中的肠杆菌属(Enterobacter)、芽孢杆菌属(Bacillus)相对丰度呈现上升趋势,不动杆菌属(Acinetobacter)、发光杆菌属(Photobacterium)相对丰度先上升后下降;池塘养殖水体中NS3a_marine_group代表的菌属的丰度先下降后上升;底泥中芽孢杆菌属(Vibrio)相对丰度变化最为明显,由最初的3.78%增加到33.64%。养殖牙鲆肠道、养殖水体和底泥中的弧菌属相对丰度在益生菌制剂添加后出现不同程度的降低。而在水产养殖中,不动杆菌属和弧菌属中的部分菌株通常被认为是重要病原菌。说明益生菌制剂的添加能够一定程度优化鱼体肠道和环境(养殖水体和池塘底泥)菌群结构。相似性分析发现,饵料不变的条件下,牙鲆肠道菌群结构与底泥的更相近;并且益生菌产品对池塘底泥和牙鲆肠道菌群的影响较为明显。相关研究结果可为池塘养殖过程中微生态制剂筛选和使用提供参考。
英文摘要:
      The deterioration of the aquaculture environment and disease often outbreaks under the high density pond culture mode of fish, probiotic/probiotics is the main and efficient method to regulate microbiota of fish intestinal tract and pond water environment, which will improve the aquaculture condition and reduce the incidence of diseases. To investigate the effects of probiotics on composition and diversity of microbiota in intestinal tract and pond culture environment of Japanese flounder (Paralichthys olivaceus), samples from Japanese flounder intestinal tract as well as environmental factors (culture water, feed and pond sediment) were collected. High throughput sequencing of 16S rDNA gene was employed to analyze the regulation of probiotics on microbiota of intestinal tract and environmental samples. Our results showed that the diversity of microbiota in pond sediment and intestinal tract of Japanese flounder were increased after adding the probiotics. Moreover, the diversity of microbiota in pond sediment was still the highest. Inversely, the diversity of microbiota in culture water was declined, which was lower than that in the intestinal tract. Firmicutes, Proteobacteria and Fusobacteria were dominant in Japanese flounder intestinal tract, after the addition of probiotics, relative abundances of Firmicutes and Proteobacteria increased while Fusobacteria decreased. At the genus level, relative abundances of Enterobacter and Bacillus increased while Acinetobacter and Photobacterium firstly increased and then decreased in Japanese flounder intestinal tract with the conduct of this experiment. Relative abundances of Chloroplast_norank increased from 42.39% to 61.44% in culture water of Japanese flounder. In pond sediment, the change of relative abundance of Bacillus was obvious, rising from 3.78% to 33.64%. Relative abundances of Vibrio in fish intestinal tract, culture water and pond sediment decreased after probiotics were added. Some strains of Acinetobacter and Vibrio are important pathogens in aquaculture. The microbiota structure in intestinal tract of Japanese flounder was more similar to that in pond sediment. Changes of these main microbiota relative abundances indicated that the addition of probiotics could optimize the microbiota structure of Japanese flounder intestinal tract and environment. However, it is impossible that Lactobacillus as the main microbiota of probiotics was able to colonize and persist in the fish intestinal tract, water and pond sediment, which indicated that probiotics deliver not the bacteria but their probiotic function. Hence, the addition of probiotics could affect the microbiota structure of Japanese flounder intestinal tract, water environment and pond sediment.
相关附件:   图片  附件1  附件2
View Fulltext   查看/发表评论  下载PDF阅读器
关闭