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| 黄海细纹狮子鱼卵巢发育的组织学观察* |
| Histological study on the ovarian development of Liparis tanakae in the Yellow Sea |
| 投稿时间：2024-07-02 修订日期：2024-08-21 |
| DOI： |
| 中文关键词: 细纹狮子鱼 组织切片 卵巢发育 卵径 |
| 英文关键词: Liparis tanakae Histological sections Ovarian development oocytes diameter |
| 基金项目:国家自然科学基金（42206104）；中国水产科学研究院黄海水产研究所基本科研业务费（20603022022022） |
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| 中文摘要: |
| 为探明黄海细纹狮子鱼(Liparis tanakae)的繁殖生物学特性,采用石蜡组织切片和HE染色法对其卵巢各发育阶段进行了组织学研究,详细描述了各时相卵母细胞的形态和卵径分布特征,并分析了细纹狮子鱼的产卵类型。结果显示,细纹狮子鱼卵母细胞发育可分为5个时相：第1时相为刚从卵原细胞分化为卵母细胞时期,细胞体积小,细胞核占比较大；第2时相卵母细胞增大,细胞周围出现一层滤泡膜；第3时相细胞质中出现卵黄泡,卵黄开始积累,质膜与滤泡膜间出现放射带,滤泡膜变为两层；第4时相卵黄开始填充卵黄泡,并逐渐结成卵黄小板；第5时相卵黄融成单一大油球,细胞核溶解。IV、V期卵巢内卵径呈单峰分布,优势粒径组分别为0.90-1.00 mm和1.70-1.80 mm,VI期卵巢卵径呈双峰分布,优势粒径组距主要集中于0.50-0.70 mm和1.10-1.20 mm,处于小生长期的卵母细胞和退化的卵母细胞比例较高。细纹狮子鱼卵巢发育模式为非同步型分批产卵类型。 |
| 英文摘要: |
| In order to investigate the reproductive biological characteristics of Liparis tanakae, histological studies of the ovaries at various developmental stages were conducted using paraffin tissue sectioning and HE staining. The morphology of the oocytes and diameter distribution characteristics of oocytes at different developmental stages were described in detail, as well as the spawning type of L. tanakae. The results showed that the development of oocyte could be divided into five stages. In stage 1, the oocyte is just differentiated from the oogonium, with small cell volume and a relatively large nucleus; the oocyte in stage 2 enlarges, and a follicle membrane appears around the cells; in stage 3, yolk vacuole start to appear in the cytoplasm of the cell and yolk begins to accumulate. Zona radiate appear between the cytoplasm membrane and the follicle membrane, and the follicle membrane becomes two-layered; in stage 4, yolk begins to fill up the yolk vacuoles, gradually forming yolk platelets; after that, yolk merges into a single large oil droplet, and the nucleus dissolves in stage 5. The sizes of the oocytes diameter in the ovaries of stage IV and V exhibited a unimodal distribution, with dominant size groups of 0.90-1.00 mm and 1.70-1.80 mm, respectively. While that in stage VI showed a bimodal distribution, with the dominant size groups concentrated on 0.50-0.70 mm and 1.10-1.20 mm, and high proportion of oocytes in the small-growth and degraded phase was observed. The result showed that L. tanakae belongs to the non-synchronized multiple batch spawner. |
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