TY - JOUR ID - TI - Evaluation of Cryopreservation Technology for ConcentratedProducts of Cholorella pyrenoidosa AU - LUO Xiaoying AU - YANG Runqing AU - WEI Dong VL - 43 IS - 1 PB - 中国水产科学研究院黄海水产研究所 SP - 172 EP - 179 PY - JF - 渔业科学进展en JA - UR - http://journal.yykxjz.cn/yykxjzen/ch/reader/view_abstract.aspx?file_no=20200930001&flag=1 KW - 蛋白核小球藻;浓缩制品;低温保藏;细胞活力;营养品质 KW - Chlorella pyrenoidosa; Concentrated products; Cryopreservation; Cell viability; Nutritional quality AB - Chlorella pyrenoidosa is widely used in aquaculture as a living food, water adjusting agent, and functional feed additive. It is thus important to study the methods of effectively maintaining cell activity and nutritional quality, and extending shelf life in the concentrated products of C. pyrenoidosa. At present, the commonly used preservation method for microalgae is cryopreservation. In this study, we used concentrated algal paste (8×1010 cells/mL) and concentrated algal fluid (1×108 cells/mL) of C. pyrenoidosa prepared by centrifugation and resuspension as the research objects, and systematically evaluated the effects of pasteurization and temperature (25℃ and 4℃) on the relative cell activity and nutrient retention rates under short-term storage without light exposure. Our results showed that pasteurization treatment caused the browning of both C. pyrenoidosa concentrates, and storage at room temperature accelerated the decay of concentrated products. Thus, the concentrated products could be stored directly at a low temperature of 4℃ for 15 days without pasteurization treatment. The total chlorophyll, carotenoids, and protein content in the concentrated C. pyrenoidosa paste reached 97.20%, 100.00%, and 98.20% of the levels before preservation, respectively; in the concentrated C. pyrenoidosa fluid all levels were 100.00% of those before preservation. This study also established the optimal conditions of the fluorescent probe method for cell viability detection: cell density of 2.6×105 cells/mL, working concentration of 60 μmol/L of fluorescein diacetate, and staining time of 30 min. The results indicated that the relative cell viability of the concentrated C. pyrenoidosa paste and fluid directly stored at 4℃ were 48.26% and 61.36%, respectively, before preservation. The cryopreservation technology established in this study for the concentrated products of C. pyrenoidosa provides a critical technology support for the downstream aquaculture applications of fresh concentrated products of microalgae. ER -