TY - JOUR ID - TI - Anesthetic effect of MS-222 and clove oil on yellowtail kingfish AU - WANG Weixin AU - CUI Aijun AU - XU Yongjiang AU - LI Haining AU - LIU Xuezhou AU - WANG Bin AU - JIANG Yan AU - LIU Xinfu VL - 42 IS - 6 PB - 中国水产科学研究院黄海水产研究所 SP - 205 EP - 214 PY - 2021/12/31/ JF - 渔业科学进展en JA - UR - http://journal.yykxjz.cn/yykxjzen/ch/reader/view_abstract.aspx?file_no=20210224001&flag=1 KW - 黄条鰤;MS-222;丁香油;麻醉效果;生理应答 KW - Yellowtail kingfish; MS-222; Clove oil; Anesthetic effect; Physiological response AB - Yellowtail kingfish Seriola aureovittata is a globally distributed, circumtropical pelagic fish of importance in both commercial fisheries and aquaculture. It swims fast and has fierce behavior under artificial culture conditions. To develop a suitable manipulation technique with low stress for yellow tail kingfish, the anesthetizing effects of two anesthetics, MS-222 and clove oil, on one-year-old yellowtail kingfish under different temperature conditions (20℃ and 24℃) were studied. The optimal anesthesia times, recovery times, and anesthesia dosages of the two anesthetics were determined based on the discrimination of swimming behavior and investigation of serum hormones levels, including cortisol, adrenaline, and glucose. According to the behavioral characteristic changes of the yellowtail kingfish during anesthesia and recovery, the anesthesia process was divided into seven stages, whereas the recovery process was divided into six stages. The results showed that the optimal concentrations for MS-222 for anesthetizing yellowtail kingfish were 100~120 mg/L and 100 mg/L at water temperatures of 20℃ and 24℃, respectively. For clove oil, the optimal concentration for anesthetizing yellowtail kingfish was 40 mg/L at both temperatures. All the experimental yellowtail kingfish were anaesthetized within three minutes and recovered within five minutes. By increasing the anesthetic concentration, the time for the yellowtail kingfish to enter anesthesia was reduced and the recovery time prolonged. The increase in water temperature reduced the time to enter anesthesia, but had no obvious effect on the recovery time. When anesthetized with 40 mg/L clove oil at 20℃ and 24℃, serum cortisol significantly peaked at 6 h and 12 h, respectively (P<0.05). At 24℃, the serum adrenaline level significantly peaked at 12 h (P<0.05) in yellowtail kingfish anesthetized with 100 mg/L of MS-222. The levels of serum adrenaline, glucose, and cortisol in the other experimental groups all significantly peaked after 24 h (P<0.05) in fish anesthetized using both anesthetics. Under the two temperature conditions, the two anesthetics caused the three serum hormone levels to all decrease significantly (P<0.05) to low levels that were lower than the initial levels after 72 h of recovery, indicating that yellowtail kingfish could be physiologically adaptive to the optimal treatment methods of clove oil and MS-222 under different temperatures. The results from the present study provide theoretical and technical support for the development of standard experimental and farming management technology for yellowtail kingfish. ER -