雨生红球藻固定化培养与虾青素累积策略的优化研究
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1.青岛科技大学;2.中国水产科学研究院黄海水产研究所;3.青岛南极维康生物科技有限公司;4.青岛科技大学化学与分子工程学院

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S968.4

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山东省博士后创新种子培养计划(SDZZ-ZR-202501182)、国家重点研发计划(2023YFD2100601)


Optimization of Immobilized Culture Techniques for Haematococcus pluvialis and Astaxanthin Accumulation Strategies
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1.Qingdao University of Science and Technology;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,;3.Qingdao Nanji Weikang Biotechnology Co., Ltd.

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    摘要:

    雨生红球藻是天然虾青素的最主要来源,但其规模化生产面临悬浮培养效率低、能耗高,以及现有固定化技术载体成本高、机械性能差、易失水等瓶颈。为构建一种高性能、低成本的固定化培养体系,本研究设计并优化了一种基于低浓度海藻酸钠的复合凝胶载体。通过系统优化氯化钙交联介质浓度、海藻酸钠与碳酸钙配比,并引入甘油作为保水剂结合聚乙烯膜覆盖技术,成功制备了兼具良好机械强度、透光性与保水性的固定化凝胶。结果表明,该最优体系在7天培养期内失水率低至49.90%,较未覆膜组降低23.10%;虾青素产量达10.787 mg,较未添加保水剂组提升32.68%。该优化体系有效解决了固定化载体易破碎、透光差、失水快的核心问题,为雨生红球藻虾青素的高效、低成本规模化生产提供了新的技术方案。

    Abstract:

    Haematococcus pluvialis is the predominant natural source of astaxanthin, yet its large-scale production is constrained by bottlenecks such as low efficiency and high energy consumption in suspension culture, as well as high cost, poor mechanical properties and easy dehydration of carriers in existing immobilization technologies. To establish a high-performance and low-cost immobilized culture system, this study designed and optimized a composite gel carrier based on low-concentration sodium alginate. By systematically optimizing the concentration of calcium chloride cross-linking medium, the mass ratio of sodium alginate to calcium carbonate, and introducing glycerol as a water-retaining agent coupled with polyethylene film covering technology, an immobilized gel with favorable mechanical strength, light transmittance and water retention capacity was successfully fabricated. The results demonstrated that within the 7-day culture period, the optimal system achieved a water loss rate as low as 49.90%, representing a 23.10% reduction compared with the non-film-covered group; the astaxanthin yield reached 10.787 mg, a 32.68% increase relative to the group without water-retaining agent. This optimized system effectively addresses the core issues of fragility, poor light transmittance and rapid water loss of immobilized carriers, providing a novel technical solution for the efficient and low-cost large-scale production of astaxanthin from Haematococcus pluvialis.

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  • 收稿日期:2026-03-24
  • 最后修改日期:2026-04-25
  • 录用日期:2026-04-29
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