| 姜燕,刘欣,徐永江,崔爱君,王滨,刘新富,柳学周,薛致勇.黄条鰤otx2和eya1基因克隆及其在早期发育阶段的表达特性.渔业科学进展,2025,46(3):27-39 |
| 黄条鰤otx2和eya1基因克隆及其在早期发育阶段的表达特性 |
| Molecular cloning and expression characteristics of otx2 and eya1 during the early development of Seriola aureovittata |
| 投稿时间：2024-04-28 修订日期：2024-05-15 |
| DOI：10.19663/j.issn2095-9869.20240428001 |
| 中文关键词: 黄条鰤 otx2 eya1 组织表达 胚胎发育 仔稚幼鱼 |
| 英文关键词: Seriola aureovittata otx2 eya1 Tissue expression Embryonic development Larva and juvenile |
| 基金项目:山东省重点研发计划(2021SFGC0701; 2024CXPT097; 2024LZGC022)、青岛海洋科技中心山东省专项经费(2022QNLM030001)、中国水产科学研究院基本科研业务费(2023TD51)和国家海水鱼产业技术体系(CARS-47)共同资助 |
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| 中文摘要: |
| 为了研究黄条鰤(Seriola aureovittata)视觉和听觉等器官关键调控基因在早期发育过程的表达特性，本研究克隆了黄条鰤 otx2和eya1的开放阅读框(ORF)序列，解析了其序列及系统进化的特性和时空表达特征。结果显示，otx2的ORF长度为876 bp，编码291个氨基酸；eya1的ORF长度为1 962 bp，编码653个氨基酸。otx2和eya1均具有广泛的组织分布特性，其中，otx2在眼组织中表达量最高，脑次之，均显著高于其余组织(P<0.05)；eya1在垂体中表达量最高，其次为卵巢，均显著高于其余组织(P<0.05)。在胚胎发育过程中均可检测到otx2和eya1的表达，且均在胚胎发育后期表达上调；其中，otx2在孵化期达到峰值，eya1在胚体包卵黄4/5期达峰值。在仔稚幼鱼时期均可追踪到otx2和eya1的表达，且在前期相对表达量较高；其中，otx2的表达呈先上调后下调的趋势，在20 dph (days post-hatching)时最高(P<0.05)，eya1的表达量呈下调趋势，在1 dph时表达量最高(P<0.05)。本研究为认识otx2和eya1在黄条鰤感知器官发育过程中可能的生理功能和研究感知器官的发育调控机制提供了分子认知基础。 |
| 英文摘要: |
| Yellowtail kingfish (Seriola aureovittata) is a pelagic migratory fish species with a global distribution. In recent years, interest in the aquaculture of this species has increased worldwide because of its high flesh quality and fast growth. As a large and fast-swimming pelagic fish, yellowtail kingfish has good adaptability to land-based industrial recirculating and offshore net cage modes. With the development of aquaculture technology, more attention has been paid to animal behavior research, which will promote the welfare level in aquaculture. Light, sound, temperature, density, and flow velocity are important factors affecting the welfare of farmed fish. The development, morphological structure, and regulatory mechanism of sensory organs, including visual, gustatory, olfactory, and auditory organs, are becoming increasingly important in the study of animal behavior.
Orthodenticle homeobox 2 (OTX2) and eye absent 1 (EYA1) play important roles in regulating the ontogeny, differentiation, and development of visual, gustatory, olfactory, and auditory organs. In order to study the expression characteristics of otx2 and eya1 in yellowtail kingfish during early development, otx2 and eya1 were identified from brain tissue with specific kits. The lengths of otx2 and eya1 open reading frame domain were 876 and 1,962 bp encoding 291 and 653 amino acids, respectively. Among these, OTX2 consists of a homologous domain from the 42nd to 93rd amino acids and a TF-Otx domain containing 82 amino acids; the C-terminal of EYA1 is an EYA domain encoding 246 amino acids. These two genes had a wide range of tissue expression characteristics, including in eye, brain, pituitary, head kidney, heart, liver, spleen, kidney, mid-gut, and ovary tissues. The highest expression level of otx2 was obtained in eye tissues followed by brain tissues, and the values in these two tissues were significantly higher than those in other tissues (P<0.05). The highest expression level of eya1 was obtained in pituitary tissues followed by ovary tissues, and the values in these two tissues were significantly higher than those in other tissues (P<0.05). During embryonic development, expression of otx2 and eya1 could be detected at each stage, and levels were upregulated at a late stage, during which otx2 and eya1 reached peak values at hatching stage and when the embryo encircled 4/5 of the yolk sac, respectively. At the larval and juvenile stages, the expression of otx2 and eya1 could be detected, with high expression levels at the early stage, during which the expression level of otx2 was first upregulated and then downregulated; the expression level at 3–25 dph (days post-hatching) was significantly higher than those at 1 dph and 30–60 dph, with the highest level reached at 20 dph (P<0.05). The expression of eya1 showed a downward trend, with the highest level reached at 1 dph (P<0.05); the expression levels at 30–60 dph were significantly lower than those at 7–25 dph (P<0.05). This study provides a molecular basis for understanding the physiological functions of otx2 and eya1 during the development of sensory organs and the regulatory mechanisms in yellowtail kingfish. |
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