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| 黄条鰤orexin及受体ox2r基因克隆、组织表达特征及其对饥饿再投喂的应答特性研究 |
| Molecular Cloning, Tissue Distribution, and Fasting-Refeeding Response of Orexin and Ox2r in yellowtail kingfish Seriola aureovittata |
| 投稿时间：2025-04-13 修订日期：2025-05-20 |
| DOI： |
| 中文关键词: 黄条鰤 orexin ox2r 组织表达 摄食 |
| 英文关键词: Seriola aureovittata orexin ox2r expression of organization feeding |
| 基金项目:中国水产科学研究院基本科研业务费(2023TD51); 农业农村部财政专项-海洋渔业生物资源收集与保藏项目；国家海洋渔业生物种质资源库项目；财政部和农业农村部：国家现代农业产业技术体系(CARS-47). |
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| 中文摘要: |
| 为探究食欲素(orexin)及其受体ox2r在黄条鰤(Seriola aureovittata)摄食调控中的作用及可能机制,获得了黄条鰤orexin和ox2r基因的开放阅读框(ORF)序列,分析了orexin和ox2r基因的组织分布、早期发育表达特征及其对饥饿再投喂的应答特性。黄条鰤orexin 基因ORF序列长450bp,编码149个氨基酸,包括46个氨基酸的信号肽、43个氨基酸的orexinA成熟肽和28个氨基酸的orexinB成熟肽。黄条鰤ox2r基因ORF序列长1269bp,编码422个氨基酸。氨基酸同源性比对发现,黄条鰤orexin和ox2r氨基酸序列在硬骨鱼类中高度保守；系统进化分析表明,黄条鰤orexin和ox2r与高体鰤的关系最近。orexin和ox2r mRNA在所检测的12种组织中均有表达,其中在脑组织表达量最高,在垂体和胃中表达量次之。在精子和卵子中可检测到orexin和ox2r mRNA的表达,表明其具有亲本遗传的特性。orexin和ox2r mRNA参与了黄条鰤胚胎及仔稚幼鱼发育过程,且在内源性营养向外源性营养转化阶段表达量变化差异显著。饥饿刺激了orexin和ox2r mRNA在脑和垂体中的上调表达,再投喂7d后恢复到对照组水平。上述结果表明orexin及受体参与了黄条鰤早期生长发育和摄食过程调控,结果为黄条鰤摄食调控机制研究积累了素材。 |
| 英文摘要: |
| The yellowtail kingfish (Seriola aureovittata) is a large, fast-swimming fish species characterized by high and aggressive feeding activity, rapid growth rate, and tender flesh. Feed intake is a key factor influencing fish growth and production performance, and appetite is a major determinant of feed intake. To explore the role and potential mechanisms of orexin and its receptor ox2r in the regulation of feeding behavior in S. aureovittata, this study employed homologous cloning to obtain the open reading frame (ORF) sequences of the orexin and ox2r genes in S. aureovittata. Quantitative real-time PCR was used to analyze the expression characteristics of these genes across various tissues and developmental stages, as well as their regulatory roles in response to starvation and subsequent refeeding. |
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