|
| 原位与异位生物絮团养殖凡纳滨对虾(Litopenaeus vannamei)的比较研究 |
| Evaluation of Integrated Effects of In-Situ and Ex-Situ Biofloc Technology for Litopenaeus vannamei aquaculture???ZHU Xinjie1,2, WANG Xiuhua2,3?, ZHENG Lei2, YANG Bing2,3, XI Rui2, PAN Mingchao2, HU Xili2 |
| 投稿时间：2025-04-21 修订日期：2025-05-05 |
| DOI： |
| 中文关键词: 原位絮团 异位絮团 凡纳滨对虾 养殖效果 综合评价 |
| 英文关键词: In-situ biofloc Ex-situ biofloc Litopenaeus vannamei Aquaculture performance Integrated assessment |
| 基金项目: |
|
| 摘要点击次数: 227 |
| 全文下载次数: 0 |
| 中文摘要: |
| 为综合比较原位与异位生物絮团在对虾养殖中的应用效果，本研究比较了在零换水条件下，原位（IB）与异位生物絮团（EB）养殖系统中，凡纳滨对虾生长性能、虾血清生理生化及免疫指标的差异，检测了养殖水体与对虾体内弧菌载量及养殖水体NH4+-N及NO2--N浓度的变化，同时分析了两种培养方式下絮团内细菌群落组成。结果表明，IB组对虾增重率与特定生长率高于EB组，饵料系数低于EB组；IB组对虾血清谷胱甘肽过氧化物酶(GSH-Px)活性高于EB组(P<0.05)，总抗氧化能力(T-AOC)低于EB组(P<0.05)。总胆固醇(T-CHO)，总蛋白(TP)，白蛋白(ALB)含量及谷草转氨酶(AST)、谷丙转氨酶(ALT)活性两个絮团组之间没有显著差异(P>0.05)；免疫指标中除IB组酚氧化酶(PO)显著活力高于EB组外(P<0.05)，两个絮团组过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及溶菌酶(LZ)活力差异不显著(P>0.05)；IB组水体中弧菌量显著低于EB组(P<0.05)，两个絮团组对虾体内弧菌差异性不显著(P>0.05)；除了在50天时IB与EB组的NH4+-N浓度差异不显著外，其他检测时段IB组NO2--N浓与NH4+-N浓度均显著低于EB组 (P<0.05)，IB组中细菌的多样性高于EB组。综上可知，原位絮团在促对虾生长、改善水质及抑制弧菌方面均优于异位絮团。研究结果为生物絮团对虾养殖模式的推广应用提供了理论支持。 |
| 英文摘要: |
| In order to comprehensively evaluate the application effects of in-situ and ex-situ biofloc technology for shrimp culture, growth performance, parameters of shrimp serum physiological, biochemical, and immunological of shrimp cultured in in-situ biofloc (IB) and ex-situ biofloc (EB) systems were investigated. A load of Vibrios in the water body and hepatopancreas of shrimp, concentrations of NH4+-N and NO2--N in aquaculture water, as well as the bacterial community composition in flocs in IB and EB systems were monitored. The results indicated that the weight gain and specific growth rates of shrimp in group IB were significantly higher than those in group EB, feed conversion ratio in group IB was lower than that in group EB. Analysis of physiological and biochemical indicators showed that the activity of serum glutathione peroxidase (GSH-Px) in IB was significantly higher than that in EB (P<0.05). Conversely, the total antioxidant capacity (T-AOC) in IB was lower than that in the EB (P<0.05), content of total cholesterol (T-CHO), total protein (TP), albumin (ALB), activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) showed no significant differences between group IB and EB(P>0.05). Activity of phenoloxidase (PO) in shrimp of group IB was higher than that in EB (P<0.05). However, the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and lysozyme (LZ) showed no significant differences between group IB and EB(P>0.05). Vibrios load in water of group IB was significantly lower than that in group EB (P<0.05), However, the difference in Vibrios load in shrimp between group IB and EB was not statistically significant (P>0.05). Concentration of NO2--N and NH4+-N in group IB were significantly lower than those in group EB, respectively, at all sampling times (P < 0.05), except for no significant difference of NH4+-N between IB and EB on day 50. Moreover, the bacterial diversity of group IB was significantly higher than that of the EB. Taken together, in-situ bioflocs demonstrated superior performance over ex-situ bioflocs in promoting shrimp growth, improving water quality, and reducing Vibrio density. These findings could provide theoretical support for the wider application of biofloc technology in shrimp aquaculture systems. |
| 附件 |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
