| 张艳珍,付龙威,隋智海,王咏星,刘云国.一株河鲈源致病性虫草菌Cordyceps confragosa CHL02菌株的全基因组测序及比较基因组分析.渔业科学进展,2021,42(4):134-144 |
| 一株河鲈源致病性虫草菌Cordyceps confragosa CHL02菌株的全基因组测序及比较基因组分析 |
| Whole-genome sequencing and comparative genome analysis of a pathogenic Cordyceps confragosa CHL02 strain isolated from Perca fluviavilis |
| 投稿时间：2020-01-15 修订日期：2020-04-10 |
| DOI： |
| 中文关键词: 虫草菌Cordyceps confragosa 全基因组测序 基因注释 比较基因组分析 |
| 英文关键词: Cordyceps confragosa Whole-genome sequencing Gene annotation Comparative genomics |
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| 中文摘要: |
| 致病性虫草菌Cordyceps confragosa CHL02菌株是从患病河鲈(Perca fluviavilis)鱼体分离鉴定的一株昆虫致病菌，其无性阶段蜡蚧轮枝菌(Lecanicillium lecanii)广泛用于农业中昆虫防治。本研究基于Illumina PE150测序平台进行CHL02菌株的全基因组测序，对测序数据进行组装和组分分析，进行基因预测与功能注释，预测次级代谢产物合成基因簇，并进行病原宿主互作以及比较基因组分析。测序结果显示，CHL02基因组大小为36.17 Mb，GC含量为53.09%；预测包含8093个编码基因、1618个转座因子(TEs)、4572个串联重复序列及114个tRNA；共注释7724个基因，其中，1985个基因获得KOG注释，GO聚类分析中，2687个基因参与代谢过程，预测到22个次级代谢产物合成基因簇，1162个基因参与病原宿主互作机制中。基因聚类分析和系统发育树均显示，CHL02菌株与参考菌株昆虫源粗糙虫草菌(C. confragosa) RCEF 1005具有较高的同源性。本研究首次报道了河鲈源致病性虫草菌C. confragosa CHL02菌株的全基因组序列并分析其基本特征，与参考菌株进行比较基因组分析，为后续深入开展该病菌侵染河鲈的作用机制等相关研究奠定理论基础。 |
| 英文摘要: |
| Cordyceps confragosa is an entomopathogenic fungus that was isolated and identified from the disease Perca fluviavilis. The anamorphic stage, Lecanicillium lecanii, has been widely used as an insect biocontrol agent in agriculture. To date, whole-genome sequencing of C. confragosa isolated from fish has never been reported. This study used the Illumina PE150 sequencing platform to whole-genome sequence CHL02 strain, and the sequencing data were assembled and analyzed by the corresponding software. Gene prediction and functional annotation were conducted, secondary metabolite synthesis gene clusters were predicted, and pathogen-host interactions and comparative genomic analyses were performed. The sequencing results showed that the CHL02 genome is 36.17 Mb with a GC content of 53.09%. There were 8093 identified genes, 1618 TEs (Transposable elements), 4572 tandem repeats, and 114 transfer RNAs (tRNAs). A total of 7724 genes were annotated, 1985 of which were obtained by KOG annotation, and 2687 genes were involved in metabolic processes in the Gene Ontology (GO) cluster analysis. Twenty-two secondary metabolite synthesis gene clusters were predicted, and 1162 genes were involved in the pathogen-host interaction mechanisms. Gene cluster analysis and the phylogenetic tree showed high homology with the reference strain C. confragosa RCEF 1005 of insect origin. This study, for the first time, reported the whole genome sequencing and comparative analysis of C. confragosa CHL02 isolated from P. fluviavilis. These results provide an important theoretical foundation for further research into the mechanisms of perch pathogen infections. |
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