The karyotype of Verasper moseri was studied through the chromosome preparations obtained from head kidney by the method of injecting PHA and colchicines. After low permeating and Carnoy’s fixation, air drying method was used to make chromosome samples, which were dyed by Giemsa afterwards.The results showed that the diploid chromosome number of Verasper moseri was 2 n=46 and its karyotype formulae were 2n=46=2 sm+44 t. Namely, there were one pair of submetacentric chromosome and 22 pairs of telocentric chromosome. NF=48.There was no visible evidence of polyploidy chromosome and sex chromosome.