In Fenneropenaeus chinesis，118 SNPs were selected for validation using high resolution melting (HRM) assays with unlabeled probes, in which 39 (33.1%) loci were polymorphic. Polymorphism of these 39 distinct loci was assessed using 48 individuals, and the results showed that the values of Ne, Ho, He and PIC varied from 1.051 2 to 1.999, from 0.000 to 0.947, from 0.049 to 0.506, from 0.049 to 0.506, respectively. The 39 contigs,to which 39 loci belonged，were annotated with Nr database. The annotation showed that, most corresponding genes were associated with immunity. The results indicated that unlabeled HRM is a simple, rapid and efficient method for SNP genotyping. These polymorphic markers will be useful genetic tools in the population genetics and breeding programs for Fenneropenaeus chinensis.