The activity of four digestive enzymes in larval and juvenile Hexagrammos otakii (0~100d) was studied. Different trends of variation were observed for the four digestive enzymes duirng the early developmental stages. The activity of pepsin gradually increased with growth and development of the fry; the activity of trypsin initially showed a gradual upward trend, though declined after reaching its peak at 20d, and then increased again after 40d and reached its maximum at 60d. The activity of amylase decreased after reaching the maximum at 20d and maintained smooth change. The activity of lipase reached a minimum at 5d and gradually increased after 40d.