Using microalgae (SA), artificial feed (AF), brachionus plicatilis (BP) and brine shrimp larvae (BS) , four diets including SA+AF, SA+AF+BP, SA+AF+BS, and SA+AF+BP+BS were formulated and used for rearing Fenneropenaeus chinensis larvae. All fertilized eggs were disinfected except the larvae fed with SA+AF. The survival rates of different treatments were compared at each stage. It was found that there was no significant difference in the survival rate at N-Z stage by feeding with different diets (P>0.05), but the survival rate of BP treatment was higher than others (P<0.01) at Z-P stage, while that of SA+AF+BS treatment was significantly different (P<0.01) from others at M-P stage. The body weight of 10-day post larvae (P10) fed with SA +AF+BP diet was less than that of SA+AF+BP+BS (P<0.01). The WSSV load of SA+AF treatment (10.52±3.3 copies/ng DNA) were significantly lower than that of other treatments (P<0.05) at P10 stage. During the stage of P11-P60, four kinds of diets, including Ruditapes philippinarum foot muscle (CF)+artificial feed (AF), R. philippinarum foot muscle radiated by 60Co γ (RCF)+artificial feed (AF), potassium-permanganate-disinfected R. philippinarum foot muscle (DCF)+artificial feed (AF) and artificial feed (AF), were used to feed the prawn. The CF+AF treatment gained the greatest increase of body weight and body length, showing no significant difference (P>0.05) from AF treatment, but with significant difference (P<0.05) from the other two treatments. The survival rate of CF+AF treatment was the highest but no significant difference from other treatments (P>0.05). WSSV artificial infection experiment showed that the accumulated mortality in all treatments were higher than 90%, with no significant difference among treatments (P>0.05) .