The eight-month experiment was conducted to determine the effects of vitamin A supplementation (0, 10000, and 80000IU/kg diet) on the growth and reproductive performance of broodstock Scophthamus maximus. Average relative fecundity, percentage of buoyant eggs, fertilization rate, hatching rate of eggs, size of eggs and length of fry were used to evaluate the performance of turbot. The results showed that the percentage of buoyant eggs and hatchability of eggs of the group fed with 80000IU/kg vitamin A were significantly higher than those of the control group(P<0.05). The body length of fry of the group fed with 80000IU/kg vitamin A was 2 684.1±24.91μm, which was significantly higher than those of the control (2 562.3±30.00μm) (P<0.05). The content of n-3HUFA and n-6HUFA in eggs and vitamin A in gonad increased with the increase of vitamin A supplementation levels in diet. SOD activity in serum of broodstock fed with 80000IU/kg vitamin A was 91.88U/ml, which was significantly higher than those of the control (70.57U/ml)(P<0.05). This experiment demonstrated that vitamin A supplemented in the diets could improve the reproductive performance of turbot broodstock.