Expression of vitellogenin (VTG) after exposure to tributyltin （TBT) was examined in hepatopancreas and ovarian of Macrobrachium rosenbergii. Results showed that the expression of VTG increased in the hepatopancreas of female M. rosenbergii, but decreased in the ovary. Expression of VTG in the hepatopancreas of male M. rosenbergii was inhibited by TBT, yet no significant effect was observed for TBT on the expression of VTG in the testis. Development of testis in M. rosenbergii was improved after exposure to TBT, but the effect of TBT on ovary development was not significant.