Portunus trituberculatus belongs to the class Crustacea, family Portunidae. It is an economically valued crab species. With the rapid expansion and intensification of Portunus trituberculatus culture, various problems such as diseases, bad quality of larvae and less natural resources, have showen and become serious, causing economic losses. These have become the limiting factors in the sustainable development of this program. At last, these problems are resolved by new varieties obatined by selective breeding. In October 2005, 1261 individuals were collected from four wild geographic populations distributed in the Yalvjiangkou, Laizhou Gulf, Zhoushan, and Haizhou Gulf . Firstly, we established fundamental stock using the four populations. Secondly we estimated the effective population size for core breeding population of P. trituberculatus, and setup breed maintenance mode. Population selection was carried out at a seed reservation rate of 5%. The new variety “Huangxuan No. 1” was selected successfully in 2010, after selection for five-generations successively. “Huangxuan No. 1” showed better economic traits such as fast growth and high survival rate. It showed a body weight increase of 20.12% and survival rate increase of 32.00% respectively, compared with the natural population. With support from the government fishery authority and crab farmers, YSFRI has made significant efforts to popularize the “Huangxuan No. 1” in Weifang, Rizhao, Qingdao and Yantai in Shandong province, Cangzhou in Hebei province, and Ningbo in Zhejiang province. The accumulated extension area during 2010—2012 was up to 6,000 ha covered by the new variety. Average yield increased 30%.The main farming model for crab is “crab,shrimp,clam and fish” ecological culture “Huangxuan No. 1” was approved by China National Aquaculture Variety Approval Committee as a new variety for aquaculture in 2012 (registered No.: GS-01-002-2012).