A new method for rapid determination of lead in fishery seawater was developed by using highly sensitive conductive carbon black paste electrode (CCBPE) as the working electrode. CCBPE showed many attractive electroanalytical properties, such as low background current, large electrochemical active area, good accumulation ability and so on. The heavy metal lead showed sensitive response at the CCBPE with anodic stripping voltammetry mode. Some important factors, including the supporting electrolyte, pH, accumulation potential, accumulation time, interference substances, etc. were studied. Under the optimized conditions, a detecting limit of 0.1 μg/L was obtained. Based on the results, a new method for determining lead in seawater was developed. The new method was used to determine the lead concentration in real seawater samples collected from nearshore site in Qingdao. It was found that the test data were in good agreement with that obtained by atomic absorption spectrometry, indicating that the simple, rapid, mercury-free and cost-effective method had a great potential value for application.