To investigate the effects of soy peptides on hepatic metabolism of juvenile starry flounder (Platichthys stellatus), two isonitrogenous (43.98±0.14% dry weight) and iso-energetic (19.89±0.08 kJ/g dry weight) experimental diets were formulated to contain 0% SPH (control, FM) and 24% SPH (SPH) respectively. Two hundred and forty juvenile starry flounders (initial weight of 5.73±0.36 g) were randomly distributed to 2 groups with three replicates per group (40 fish/replicate). Each group was fed one diet for 50 days before collecting liver samples for analysis. The results showed that 448−470 protein spots were detected in the gel area of pH 4−7 and Mr 14.4−97.4 kDa after 2-dimensional gel electrophoresis. Among them, fourteen hepatic proteins showed over 2-fold difference in expression abundance including 3 structural proteins (intermediate filament protein ON3-like, type II cytoskeletal 8-like isoform X2 and β-actin) and 11 proteins involved in different metabolism. Nine metabolic proteins and 3 structural proteins were up-regulated by at least 2-fold and other 2 metabolic proteins were decreased by 2-fold in fish fed SPH diet compared those fed FM diet. The present results revealed that SPH affected hepatic metabolism by enhancing synthesis of high density lipoprotein and stress protein, promoting glycolysis metabolism for energy production while reducing synthesis of trypsin. The present study elucidated the global metabolic response of fish liver to soy peptides using comparative proteomics technology and provides a theoretical basis to apply the novel plant proteins in aquatic feed.