High scatter ratio (ScR) of feed diets and the lack of effective disease prevention and control method have been hindering the cage culture of sea cucumbers (Apostichopus japonicus). In this study we used adhesive feed with bio-gel and 3 types of feed supplements (Enteromorpha, microecologics and Chinese herbal medicine) to evaluate their effects on the growth and survival of the cultured sea cucumbers. We designed two categories of feed (adhesive feed with 20% bio-gel and control feed without bio-gel), and divided them into eight groups including the blank adhesive feed (AF-b), the enteromorpha adhesive feed (AF-en), the microecologics adhesive feed (AF-mi), the Chinese herbal medicine adhesive feed (AF-h), the blank control feed (CF-b), the enteromorpha control feed (CF-en), the microecologics control feed (CF-mi), and the Chinese herbal medicine control feed (CF-h). The survival rates (SR), the weight gain rates (WGR), the specific growth rates, the activities of protease, amylase, cellulose, and the microstructure of foregut were measured as the indicators of the effects of feed supplements during the 42-day feeding experiment. The average ScR of the adhesive feed groups (AF-b, AF-en, AF-mi, and AF-h) was reduced by 33.42% compared to the control groups (CF-b, CF-en, CF-mi, and CF-h). There was no significant difference between the 3 groups with food supplements (P>0.05). The WGR and SGR of AF-h were (41.50±1.39)% and (0.82±0.02) %/d respectively, which were the highest among all groups and the values were significantly different from other 4 groups (CF-b, AF-b, AF-en, and AF-mi) (P<0.05). The SR of AF-h (94.03%±2.28%) was the highest among all groups. The SRs of AF-h and AF-mi were significantly higher than that of CF-b and AF-b (P<0.05). The activity of amylase of AF-en, AF-mi and AF-h reached the maximum on the 10th, 20th and 30th day during the experiment, and the peak values were (1.70±0.05) U∙mg prot-1, (1.60±0.04) U∙mg prot-1 and (1.77±0.04) U∙mg prot-1 respectively. Compared to the other 4 groups, the activity of protease of AF-b fluctuated tremendously and reached (1.78±0.09) U·mg prot-1 on the 10th day during the experiment. The activity of cellulose of CF-b, AF-en and AF-b first increased during the early stage and decreased later; the activity of cellulose of AF-h continued to increase during the entire experiment; the activity of cellulose of AF-mi decreased during the first 20 days and then increased in the later 20 days. The microstructures of the foregut of the 5 groups (CF-b, AF-b, AF-en, AF-mi, and AF-h) were normal. The microscopic observation revealed an intense secretion activity of the epithelial cells in AF-mi and AF-h. The relative thickness of the mucosa of AF-b and the muscular layer of AF-en increased significantly (P<0.05). These results indicated that the adhesive feed with bio-gel could effectively decrease ScR of feed diets, and that WGR, SR and digestion activities could be increased with the addition of microecologics and Chinese herbal medicine. Furthermore, Enteromorpha did not affect the growth of cage-cultured sea cucumbers, which indicated that it could be used as a substitute in the feed diets for sea cucumbers.