| 刘长琳,庄志猛,陈四清,邹健,李学文.星突江鲽精子的超微结构.渔业科学进展,2009,30(4):14-20 |
| 星突江鲽精子的超微结构 |
| Ultra-structure of the spermatozoon of Platichthys stellatus |
| 投稿时间：2008-10-08 修订日期：2009-01-15 |
| DOI： |
| 中文关键词: 星突江鲽 精子 超微结构 |
| 英文关键词: Platichthys stellatus Spermatozoon Ultra structure |
| 基金项目:国家科技支撑计划项目（2006BAD03B08 07）、黄海水产研究所基本科研业务项目（2060302/2）和青岛市科技发展计划项目（06-2-3-18-HY）共同资助 |
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| 中文摘要: |
| 应用扫描电镜和透射电镜观察了星突江鲽Platichthys stellatus精子的超微结构。星突江鲽的精子由头部、中段和尾部组成。头部呈球形，主要结构为细胞核。核前端无顶体，后端有植入窝，植入窝较浅，凹入深度约为细胞核直径的1/4。核中染色质致密，但存在染色质较浅的头部凹陷和核泡。中段包括中心粒复合体和袖套。近端中心粒的长轴与基体的长轴相互垂直，呈“T”字型排列，基体的长轴与精子的长轴平行。袖套很浅，其最深处只达到线粒体下端的1/3处。在袖套线粒体的外面，具有大量的椭圆形囊泡存在。尾部细长，主要由轴丝构成，为典型的“9+2”微管结构。鞭毛较粗，长有两排侧鳍，侧鳍不对称。鞭毛细胞质中可见大小不一的椭圆形囊泡存在，囊泡的分布不均匀，一侧囊泡数量较多，另一侧偏少。鞭毛的这种结构与精子的运动相适应。 |
| 英文摘要: |
| Ultra structure of the spermatozoon of Platichthys stellatus was observed by scanning and transmission electron microscopy. The spermatozoon of P. stellatus consisted of a head, a midpiece and a tail. The head was spherical in shape, of which the main structure was the nucleus. It had no acrosome but had an implantation fossa at its caudal end. The implantation fossa was shallow, with the depth of 1/4 of the nucleus diameter. The nucleus consisted of electron dense chromatin materials, with a head pit and nuclear vacuole whose chromatin was free. The midpiece consisted of the centriolar complex and the sleeve. The proximal centriole and the basal body were arranged at an orthogonal, and in “T” structure, while the basal body was parallelled to the spermatozoon. The sleeve was very shallow, and the deepest point was just 1/3 to the mitochondrion. There were many ellipse vesicles in the sleeve outside mitochondrion. The main structure of the tail was axoneme, which was slim and long. The structure of the tail was of the typical “9+2” model. The flagella were thick, and there were two rows of lateral fins on the flagellum, but the fin was not asymmetrical. There were many different sizes of ellipse vesicles in the flagella cytoplasm, which were of asymmetry. The structure of the flagellum was accommodated for the spermatozoon move. |
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