| 田永胜,陈松林,季相山,孙礼娟,翟介明.半滑舌鳎精子冷冻保存.渔业科学进展,2009,30(6):97-102 |
| 半滑舌鳎精子冷冻保存 |
| Cryopreservation of tongue sole Cynoglossus semilaevis sperm |
| 投稿时间：2008-12-18 修订日期：2009-04-07 |
| DOI： |
| 中文关键词: 半滑舌鳎 精子 冷冻保存 |
| 英文关键词: Cynoglossus semilaevis Semen Cryopreservation |
| 基金项目:国家自然科学基金项目（30570259）、“国家”863项目(2006AA10A403)、农业科技成果转化资金项目（2007GB2C600174）和农业部海洋渔业资源可持续利用重点开放实验室开放课题（实开2005-03）共同资助 |
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| 中文摘要: |
| 半滑舌鳎精子冷冻保存对于人工繁殖育苗、杂交育种、雌核发育及其性别控制研究具有重要的意义，为此，本文对半滑舌鳎精子冷冻保存方法进行了研究。分别利用2.8 mol/L的二甲基亚砜（DMSO）、甘油（Gly）和1，2-丙二醇（PG）冷冻保存该鱼精子。结果显示，DMSO冷冻保存精子的活力较高。利用MPRS+2.8 mol/L DMSO以1∶05、1∶1、1∶1.5和1∶2的比例稀释并冷冻精子，1∶1比例在冻前能够抑制精子的运动，冻后活力可达82.50±3.54%，显著高于其他稀释比例（P<0.05）。分别利用冷冻保存液A（MPRS+2.8 mol/L DMSO）和B（TS-2+2.8 mol/L DMSO）稀释平衡精子，精子在A中的冻前快速运动时间、寿命分别为37.75±6.45 s和145.00±78.98 s，与鲜精无显著差异（P>0.05）。利用以上两种冷冻稀释液冷冻保存精子，精子在A液中的冻后活力和寿命分别可达53.50±6.69%和98.00±13.51 s，冷冻效果优于B液（P<0.05）。冷冻后精子的受精率和孵化率分别为55.00±5.00%和35.00±13.23%，受精率与鲜精无显著性差异（P>0.05），因此认为MPRS+2.8 mol/L DMSO可用于半滑舌鳎精子的冷冻保存。 |
| 英文摘要: |
| Cryopreservation of tongue sole Cynoglossus semilaevis sperm offers benefits for artificial propagation, hybridization, gynogenesis and sex control.In order to develop a protocol to freeze tongue sole sperm,the effects of cryoprotectants, extenders and dilution ratio on motility,fertilization rate and hatching rate of frozen-thawed sperm were evaluated. Dimethylsulfoxide(DMSO)provided higher protection than glycerol and 1,2 propylene glycol during freezing and thawing.Motion time and life of sperm in A(MPRS +28 mol/L DMSO)and B(TS-2+2.8 mol/L DMSO)before freezing were 37.75±6.45 sec and 145.00±78.98 sec,which was not different from that of fresh sperm(P>0.05).The motility and life of frozen thawed sperm in A were 5350±669% and 98.00±13.51 sec,which was significantly higher than that of frozen thawed sperm in B.When sperm were diluted with A at a ratio of 1∶05,1∶1,1∶15 and 1∶2, the highest motility(82.5±3.54%, 1∶1 dilution ratio)of frozen-thawed sperm was obtained and which was significantly higher than that of other dilution ratio treatment.According to the results above, tongue sole sperm were cryopreserved successfully with MPRS+2.8 mol/L DMSO at a dilution ratio of 1∶1.As a result,the fertilization rate(55.0±5.0%) and hatching rate(35±13.23%) of frozen-thawed sperm were obtained,which was not significantly different from that of fresh sperm(P>0.05). |
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