| 周丽青,杨爱国,吴彪,程鹏,于宏,王华,雷霁霖.波纹唇鱼染色体制备及核型的初步研究.渔业科学进展,2010,31(1):54-58 |
| 波纹唇鱼染色体制备及核型的初步研究 |
| A preliminary study on chromosome preparation and karyotype |
| 投稿时间：2009-04-16 修订日期：2009-06-30 |
| DOI： |
| 中文关键词: 波纹唇鱼 染色体 鳍条 愈合增生组织 核型 |
| 英文关键词: Humphead wrasse Cheilinus undulatus Chromosome Fin Healing hyperplastic tissue Karyotype |
| 基金项目:国家“863”计划项目（2006AA10A414）资助 |
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| 中文摘要: |
| 出于对波纹唇鱼这一濒危物种资源调查和保护的目的，探讨了活体状况下波纹唇鱼染色体制备的方法，并对其染色体进行分析。剪取1龄波纹唇鱼的微量尾部鳍条及对应部位的愈合增生组织为材料，采用热滴片法制备染色体标本。核型分析结果表明，波纹唇鱼二倍体染色体数目2n=48，核型为2n=4M +10SM + 32ST +2T，因所取鱼的条数有限，且均为雌性，所以尚不能确定是否有异型性染色体的存在。 |
| 英文摘要: |
| In order to protect the humphead wrasse Cheilinus undulatus (Ruppell, 1835）, an endangered species，the metaphase chromosome preparations of the live fish and its karyotype were studied in this paper. The metaphase chromosome was obtained from the fin and its healing-tissue of one-year old fish by hot air drying methods. The results showed that there were 48 chromosomes in diploid and their karyotype formulae was 2n=4M +10SM + 32ST +2T.Heterotypic sex chromosome was not found since the number of the tested fish was limited and all the young fish were physiologically female. |
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