| 郭奇,魏玉西,殷邦忠,刘淇,刘蕾.鼠尾藻多酚分级组分的抑菌活性研究.渔业科学进展,2010,31(1):117-121 |
| 鼠尾藻多酚分级组分的抑菌活性研究 |
| Study on the antimicrobial activity of different fractions of phlorotannins from Sargassum thunbergii kuntze |
| 投稿时间：2009-10-24 修订日期：2009-11-26 |
| DOI： |
| 中文关键词: 鼠尾藻 褐藻多酚 抑菌活性 |
| 英文关键词: Sargassum thunbergii kuntze Phlorotannins Antibacterial activity |
| 基金项目:公益性行业（农业）专项(2009030300) 和青岛市科技计划项目(No.06 2212 JCH)共同资助 |
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| 中文摘要: |
| 采用平板生长抑制法对鼠尾藻 Sargassum thunbergii kuntze 多酚化合物各分级组分进行抑菌活性研究。结果表明，在一定浓度范围内各分级组分对副溶血弧菌、哈维氏弧菌、沙蚕弧菌、金黄色葡萄球菌、大肠杆菌、鳗弧菌、溶藻弧菌和四联微球菌等受试菌株均有抑菌活性。其中，分级组分I (Mr < 5.0×103)的抑菌效果优于其他组分，对副溶血弧菌、哈维氏弧菌和沙蚕弧菌的最低抑菌浓度(MIC)值为900 μg/ml，对金黄色葡萄球菌、大肠杆菌、鳗弧菌和溶藻弧菌的MIC值为1 800 μg/ml，对四联微球菌的MIC值为3 600 μg/ml。另外，组分I (Mr < 5.0×103)显示出较好的热稳定性，在pH 3～4时对受试菌的抑菌活性最强。 |
| 英文摘要: |
| The antimicrobial activity of different molecular-weight fractions of phlorotannins from Sargassum thunbergii kuntze was screened by plate growth inhibition assay. The results indicated that all the fractions of phlorotannins can inhibit the growth of tested microorganisms including Vibrio parahaemolyticus, Vibrio harveyi, Vibrio nereis, Staphylococcus aureus, Escherichia coli, Vibrio anguillarum, Vibrio alginolyticus and Micrococcus tetragenus. Among them, the antibacterial activity of the lowest molecular-weight fraction I (Mr < 5.0×103) of phlorotannins was the best with the minimum inhibition concentration (MIC) values against the tested microorganisms above as 900，900，900，1 800，1 800，1 800，1 800 and 3 600 μg/ml respectively. Additionally, phlorotannins from S.thunbergii kuntze showed excellent thermal stability, and the best antimicrobial activity to the tested bacteria was in pH 3～4. |
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