Static method was employed to study feeding behaviors of Japanese scallop Patinopecten yessoensis feeding on different food such as flounder Paralichthys olivaceus feces, residual feed, sediment particulates and microalgae.Clearance rate and ingestion rate on microalgae were significantly higher than the other three experimental groups (ANOVA, P<0.01). Absorption efficiencies (AE) among microalgae, fish feces and residual feed were not significantly different, but they were significantly higher than that on sediment particulates. There was a positive correlation between AE and organic content (f): AE(％)＝38.84lnf+72.7 (R2=0.378); The linear relationship between absorption rate (AR) and particulate organic matter (POM) was described as: AR＝0.118×POM-0.555 (R2=0.329). Total particulate matter (TPM) threshold for pseudo-feces production was 24.09 mg/L, and the scallop could regulate food intake by pseudo-feces production. Based on the research results,the potential Integrated Multi-trophic Aquaculture (IMTA)of shellfish and fish was proposed.